...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Experimental Botany >Polyols in grape berry: transport and metabolic adjustments as a physiological strategy for water-deficit stress tolerance in grapevine
【24h】

Polyols in grape berry: transport and metabolic adjustments as a physiological strategy for water-deficit stress tolerance in grapevine

机译：葡萄浆果中的多元醇：运输和代谢调节作为葡萄缺水胁迫耐受性的生理策略

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Polyols are important metabolites that often function as carbon and energy sources and/or osmoprotective solutes in some plants. In grapevine, and in the grape berry in particular, the molecular aspects of polyol transport and metabolism and their physiological relevance are virtually unknown to date. Here, the biochemical function of a grapevine fruit mesocarp polyol transporter (VvPLT1) was characterized after its heterologous expression in yeast. This H+-dependent plasma membrane carrier transports mannitol (K-m=5.4 mM) and sorbitol (K-m=9.5 mM) over a broad range of polyols and monosaccharides. Water-deficit stress triggered an increase in the expression of VvPLT1 at the fully mature stage, allowing increased polyol uptake into pulp cells. Plant polyol dehydrogenases are oxireductases that reversibly oxidize polyols into monosaccharides. Mannitol catabolism in grape cells (K-m=30.1 mM mannitol) and mature berry mesocarps (K-m=79 mM) was, like sorbitol dehydrogenase activity, strongly inhibited (50-75%) by water-deficit stress. Simultaneously, fructose reduction into polyols via mannitol and sorbitol dehydrogenases was stimulated, contributing to their higher intracellular concentrations in water-deficit stress. Accordingly, the concentrations of mannitol, sorbitol, galactinol, myo-inositol, and dulcitol were significantly higher in berry mesocarps from water-deficit-stressed Tempranillo grapevines. Metabolomic profiling of the berry pulp by GC-TOF-MS also revealed many other changes in its composition induced by water deficit. The impact of polyols on grape berry composition and plant response to water deficit stress, via modifications in polyol transport and metabolism, was analysed by integrating metabolomics with transcriptional analysis and biochemical approaches.
机译：多元醇是重要的代谢产物，在某些植物中通常充当碳和能源和/或渗透保护性溶质。迄今为止，在葡萄树中，尤其是在葡萄浆果中，多元醇运输和代谢的分子方面及其生理相关性实际上是未知的。在这里，葡萄果中果皮多元醇转运蛋白（VvPLT1）的生化功能是在酵母中异源表达后表征的。这种依赖于H +的质膜载体可在多种多元醇和单糖上运输甘露醇（K-m = 5.4 mM）和山梨糖醇（K-m = 9.5 mM）。缺水胁迫在完全成熟阶段触发了VvPLT1表达的增加，从而使多元醇对果肉细胞的吸收增加。植物多元醇脱氢酶是将多元醇可逆地氧化成单糖的氧化还原酶。像脱水山梨糖醇脱氢酶活性一样，水分缺乏胁迫对葡萄细胞（K-m = 30.1 mM甘露醇）和成熟浆果中果皮（K-m = 79 mM）中的甘露醇分解代谢产生了强烈的抑制作用（50-75％）。同时，果糖通过甘露醇和山梨糖醇脱氢酶还原成多元醇被刺激，导致它们在缺水胁迫下的细胞内浓度更高。因此，在缺水胁迫的坦普拉尼约葡萄的浆果中果皮中，甘露醇，山梨糖醇，半乳糖醇，肌醇和二甘醇的浓度明显更高。 GC-TOF-MS对浆果果肉进行代谢组学分析也显示了水分缺乏引起的其成分的许多其他变化。通过将代谢组学与转录分析和生化方法相结合，分析了多元醇对葡萄浆果组成和植物对水分亏缺胁迫的反应的影响，方法是通过多元醇运输和代谢的修饰。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号