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首页> 外文期刊>Journal of Experimental Botany >A wheat ABC transporter contributes to both grain formation and mycotoxin tolerance
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A wheat ABC transporter contributes to both grain formation and mycotoxin tolerance

机译：小麦ABC转运蛋白有助于谷物形成和霉菌毒素耐受性

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Genes that enhance resistance to the Fusarium virulence factor deoxynivalenol (DON) are targets for disease resistance breeding. This study provides direct evidence that a wheat ABCC3 enhances resistance to DON.The mycotoxin deoxynivalenol (DON) acts as a disease virulence factor for Fusarium fungi, and tolerance of DON enhances wheat resistance to Fusarium head blight (FHB) disease. Two variants of an ATP-binding cassette (ABC) family C transporter gene were cloned from DON-treated wheat mRNA, namely TaABCC3.1 and TaABCC3.2. These represent two of three putative genes identified on chromosomes 3A, 3B, and 3D of the wheat genome sequence. Variant TaABCC3.1 represents the DON-responsive transcript previously associated with DON resistance in wheat. PCR-based mapping and in silico sequence analyses located TaABCC3.1 to the short arm of wheat chromosome 3B (not within the FHB resistance quantitative trait locus Fhb1). In silico analyses of microarray data indicated that TaABCC3 genes are expressed in reproductive tissue and roots, and in response to the DON producer Fusarium graminearum. Gene expression studies showed that TaABCC3.1 is activated as part of the early host response to DON and in response to the FHB defence hormone jasmonic acid. Virus-induced gene silencing (VIGS) confirmed that TaABCC3 genes contributed to DON tolerance. VIGS was performed using two independent viral construct applications: one specifically targeted TaABCC3.1 for silencing, while the other targeted this gene and the chromosome 3A homeologue. In both instances, VIGS resulted in more toxin-induced discoloration of spikelets, compared with the DON effects in non-silenced spikelets at 14 d after toxin treatment (a parts per thousand yen2.2-fold increase, P 0.05). Silencing by both VIGS constructs enhanced head ripening, and especially so in DON-treated heads. VIGS of TaABCC3 genes also reduced the grain number by more than 28% (P 0.05), both with and without DON treatment, and the effects were greater for the construct that targeted the two homeologues. Hence, DON-responsive TaABCC3 genes warrant further study to determine their potential as disease resistance breeding targets and their function in grain formation and ripening.
机译：增强对镰刀菌毒力因子脱氧雪腐酚（DON）的抗性的基因是抗病育种的目标。这项研究提供了直接的证据，证明小麦ABCC3增强了对DON的抗性。霉菌毒素脱氧雪腐烯酚（DON）是镰刀菌真菌的致病因子，而DON的耐受性则增强了小麦对镰刀菌病的抵抗力。从DON处理过的小麦mRNA中克隆了ATP结合盒（ABC）C家族转运蛋白基因的两个变体，即TaABCC3.1和TaABCC3.2。这些代表在小麦基因组序列的染色体3A，3B和3D上鉴定的三个推定基因中的两个。 TaABCC3.1变体代表以前与小麦中DON抗性相关的DON响应转录本。基于PCR的作图和计算机序列分析将TaABCC3.1定位于小麦3B染色体的短臂（不在FHB抗性定量性状基因座Fhb1内）。对微阵列数据的计算机分析表明，TaABCC3基因在生殖组织和根中表达，并响应DON生产者禾谷镰刀菌。基因表达研究表明，TaABCC3.1作为早期宿主对DON和FHB防御激素茉莉酸的响应而被激活。病毒诱导的基因沉默（VIGS）证实TaABCC3基因有助于DON耐受。使用两种独立的病毒构建体应用程序进行了VIGS：一种专门针对TaABCC3.1沉默，而另一种针对该基因和3A染色体同源。在这两种情况下，与在毒素处理后14 d的非沉默小穗中的DON效应相比，VIGS导致更多的毒素诱导小穗变色（每千日元的含量增加2.2倍，P <0.05）。两个VIGS构造的沉默都增强了头部成熟，尤其是在DON处理过的头部中更是如此。 TaABCC3基因的VIGS在有和没有DON处理的情况下，也都使晶粒数减少了28％以上（P <0.05），并且针对两种同源基因的构建体的效果更大。因此，DON反应性TaABCC3基因值得进一步研究，以确定其作为抗病育种目标的潜力及其在谷物形成和成熟中的功能。

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