...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Experimental Botany >Tissue-specific root ion profiling reveals essential roles of the CAX and ACA calcium transport systems in response to hypoxia in Arabidopsis
【24h】

Tissue-specific root ion profiling reveals essential roles of the CAX and ACA calcium transport systems in response to hypoxia in Arabidopsis

机译：组织特异性根离子图谱揭示了拟南芥低氧反应中CAX和ACA钙转运系统的重要作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Waterlogging is a major abiotic stress that limits the growth of plants. The crucial role of Ca2+ as a second messenger in response to abiotic and biotic stimuli has been widely recognized in plants. However, the physiological and molecular mechanisms of Ca2+ distribution within specific cell types in different root zones under hypoxia is poorly understood. In this work, whole-plant physiological and tissue-specific Ca2+ changes were studied using several ACA (Ca2+-ATPase) and CAX (Ca2+/proton exchanger) knock-out Arabidopsis mutants subjected to waterlogging treatment. In the wild-type (WT) plants, several days of hypoxia decreased the expression of ACA8, CAX4, and CAX11 by 33% and 50% compared with the control. The hypoxic treatment also resulted in an up to 11-fold tissue-dependent increase in Ca2+ accumulation in root tissues as revealed by confocal microscopy. The increase was much higher in stelar cells in the mature zone of Arabidopsis mutants with loss of function for ACA8, ACA11, CAX4, and CAX11. In addition, a significantly increased Ca2+ concentration was found in the cytosol of stelar cells in the mature zone after hypoxic treatment. Three weeks of waterlogging resulted in dramatic loss of shoot biomass in cax11 plants (67% loss in shoot dry weight), while in the WT and other transport mutants this decline was only 14-22%. These results were also consistent with a decline in leaf chlorophyll fluorescence (Fv/Fm). It is suggested that CAX11 plays a key role in maintaining cytosolic Ca2+ homeostasis and/or signalling in root cells under hypoxic conditions.
机译：涝渍是限制植物生长的主要非生物胁迫。 Ca 2+作为响应非生物和生物刺激的第二信使的关键作用已在植物中得到广泛认可。但是，对缺氧条件下不同根区特定细胞类型中Ca2 +分布的生理和分子机制了解甚少。在这项工作中，使用数个经过浸水处理的ACA（Ca2 + -ATPase）和CAX（Ca2 + /质子交换器）敲除拟南芥突变体研究了全植物生理和组织特定的Ca2 +变化。与对照相比，在野生型（WT）植物中，缺氧数天使ACA8，CAX4和CAX11的表达降低了33％和50％。共聚焦显微镜显示，低氧处理还导致根组织中Ca2 +积累的组织依赖性增加最多11倍。在拟南芥突变体的成熟区，对ACA8，ACA11，CAX4和CAX11功能丧失的硬皮细胞中，这种增加要高得多。另外，在低氧处理后的成熟区中，在石质细胞的胞质溶胶中发现钙离子浓度显着增加。三周的涝渍导致cax11植物的茎生物量急剧减少（茎干重减少67％），而在WT和其他运输突变体中，这种下降仅为14-22％。这些结果也与叶绿素荧光（Fv / Fm）的下降一致。提示在缺氧条件下，CAX11在维持细胞内Ca2 +稳态和/或根细胞信号传导中起关键作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号