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首页> 外文期刊>Journal of Experimental Botany >The transcription factor AREB1 regulates primary metabolic pathways in tomato fruits.
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The transcription factor AREB1 regulates primary metabolic pathways in tomato fruits.

机译：转录因子AREB1调节番茄果实中的主要代谢途径。

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Tomato fruit development is regulated both by the action of plant hormones and by tight genetic control. Recent studies suggest that abscisic acid (ABA) signalling may affect different aspects of fruit maturation. Previously, it was shown that SlAREB1, an ABA-regulated transcription factor involved in stress-induced responses, is expressed in seeds and in fruit tissues in tomato. Here, the role of SlAREB1 in regulating the expression of genes relevant for primary metabolic pathways and affecting the metabolic profile of the fruit was investigated using transgenic tomato lines. Metabolite profiling using gas chromatography-time of flight mass spectrometry (GC-TOF-MS) and non-targeted liquid chromatography-mass spectrometry (LC-MS) was performed on pericarp tissue from fruits harvested at three stages of fruit development. Principal component analysis of the data could distinguish the metabolite profiles of non-transgenic fruits from those that overexpress and down-regulate SlAREB1. Overexpression of SlAREB1 resulted in increased content of organic acids, hexoses, hexose-phosphates, and amino acids in immature green, mature green, and red ripe fruits, and these modifications correlated with the up-regulation of enzyme-encoding genes involved in primary carbohydrate and amino acid metabolism. A non-targeted LC-MS analysis indicated that the composition of secondary metabolites is also affected in transgenic lines. In addition, gene expression data revealed that some genes associated with fruit ripening are also up-regulated in SlAREB1-overexpressing lines compared with wild-type and antisense lines. Taken together, the results suggest that SlAREB1 participates in the regulation of the metabolic programming that takes place during fruit ripening and that may explain part of the role of ABA in fruit development in tomato
机译：番茄果实的发育受植物激素的作用和严格的基因控制。最近的研究表明，脱落酸（ABA）信号可能会影响水果成熟的不同方面。以前的研究表明，SalAREB1是一种ABA调控的转录因子，参与胁迫诱导的应答，在番茄的种子和果实组织中表达。在这里，使用转基因番茄品系研究了SlAREB1在调节与主要代谢途径有关的基因表达并影响果实代谢特性中的作用。对在果实发育三个阶段收获的果实的果皮组织进行了气相色谱-飞行时间质谱分析（GC-TOF-MS）和非目标液相色谱-质谱分析（LC-MS）代谢物分析。数据的主成分分析可以将非转基因水果的代谢物谱与过量表达和下调SlAREB1的水果相区别。 SlAREB1的过表达导致未成熟的绿色，成熟的绿色和红色成熟的水果中有机酸，己糖，己糖磷酸盐和氨基酸的含量增加，并且这些修饰与初级碳水化合物中涉及的酶编码基因的上调相关和氨基酸代谢。非靶向LC-MS分析表明，次级代谢产物的组成在转基因品系中也受到影响。此外，基因表达数据显示，与野生型和反义品系相比，一些与果实成熟相关的基因在SLAREB1过表达品系中也被上调。两者合计，结果表明SlAREB1参与了果实成熟过程中发生的代谢程序调节，可能解释了ABA在番茄果实发育中的部分作用

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