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首页> 外文期刊>Journal of Experimental Botany >Wheat TaNPSN SNARE homologues are involved in vesicle-mediated resistance to stripe rust ( Puccinia striiformis f. sp. tritici).
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Wheat TaNPSN SNARE homologues are involved in vesicle-mediated resistance to stripe rust ( Puccinia striiformis f. sp. tritici).

机译：小麦TaNPSN SNARE同源物与水泡介导的抗条锈病有关（Puccinia striiformis f。sp。tritici）。

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摘要

Subcellular localisation of SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) and their ability to form SNARE complexes are critical for determining the specificity of vesicle fusion. NPSN11, a Novel Plant SNARE ( NPSN) gene, has been reported to be involved in the delivery of cell wall precursors to the newly formed cell plate during cytokinesis. However, functions of NPSN genes in plant-pathogen interactions are largely unknown. In this study, we cloned and characterized three NPSN genes ( TaNPSN11, TaNPSN12, and TaNPSN13) and three plant defence-related SNARE homologues ( TaSYP132, TaSNAP34, and TaMEMB12). TaSYP132 showed a highly specific interaction with TaNPSN11 in both yeast two-hybrid and bimolecular fluorescence complementation (BiFC) assays. We hypothesize that this interaction may indicate a partnership in vesicle trafficking. Expressions of the three TaNPSNs and TaSYP132 were differentially induced in wheat leaves when challenged by Puccinia striiformis f. sp. tritici ( Pst). In virus-induced gene silencing (VIGS) assays, resistance of wheat ( Triticum aestivum) cultivar Xingzi9104 to the Pst avirulent race CYR23 was reduced by knocking down TaNPSN11, TaNPSN13 and TaSYP132, but not TaNPSN12, implying diversified functions of these wheat SNARE homologues in prevention of Pst infection and hyphal elongation. Immuno-localization results showed that TaNPSN11 or its structural homologues were mainly distributed in vesicle structures near cell membrane toward Pst hypha. Taken together, our data suggests a role of TaNPSN11 in vesicle-mediated resistance to stripe rust. RI Dubcovsky, Jorge/A-4969-2008
机译：SNARE（可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体）的亚细胞定位及其形成SNARE复合物的能力对于确定囊泡融合的特异性至关重要。 NPSN11，一种新型植物SNARE（NPSN）基因，据报道参与胞质分裂过程中细胞壁前体向新形成的细胞板的传递。但是，NPSN基因在植物与病原体相互作用中的功能尚不清楚。在这项研究中，我们克隆并鉴定了三个NPSN基因（TaNPSN11，TaNPSN12和TaNPSN13）和三个植物防御相关的SNARE同源物（TaSYP132，TaSNAP34和TaMEMB12）。 TaSYP132在酵母双杂交和双分子荧光互补（BiFC）分析中均显示出与TaNPSN11的高度特异性相互作用。我们假设这种相互作用可能表明小泡贩运中的伙伴关系。当小麦条锈菌对小麦叶片中的三种TaNPSNs和TaSYP132的表达有差异。 sp。小麦（Pst）。在病毒诱导的基因沉默（VIGS）分析中，通过敲除TaNPSN11，TaNPSN13和TaSYP132而不是TaNPSN12可以降低小麦（Triticum aestivum）品种Xingzi9104对Pst无毒小种CYR23的抗性，这表明这些小麦SNARE同系物的功能多样化。预防Pst感染和菌丝伸长。免疫定位结果表明，TaNPSN11或其结构同源物主要分布在细胞膜附近向Pst菌丝的囊泡结构中。两者合计，我们的数据表明TaNPSN11在囊泡介导的抗条锈病中发挥作用。 RI杜布科夫斯基，豪尔赫/ A-4969-2008

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