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首页> 外文期刊>Journal of Experimental Botany >The nucleolar GTPase nucleostemin-like 1 plays a role in plant growth and senescence by modulating ribosome biogenesis
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The nucleolar GTPase nucleostemin-like 1 plays a role in plant growth and senescence by modulating ribosome biogenesis

机译：核仁GTPase核蛋白样1通过调节核糖体生物发生在植物生长和衰老中起作用

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摘要

Nucleostemin is a nucleolar GTP-binding protein that is involved in stem cell proliferation, embryonic development, and ribosome biogenesis in mammals. Plant nucleostemin-like 1 (NSN1) plays a role in embryogenesis, and apical and floral meristem development. In this study, a nucleolar function of NSN1 in the regulation of ribosome biogenesis was identified. Green fluorescent protein (GFP)-fused NSN1 localized to the nucleolus, which was primarily determined by its N-terminal domain. Recombinant NSN1 and its N-terminal domain (NSN1-N) bound to RNA in vitro. Recombinant NSN1 expressed GTPase activity in vitro. NSN1 silencing in Arabidopsis thaliana and Nicotiana benthamiana led to growth retardation and premature senescence. NSN1 interacted with Pescadillo and EBNA1 binding protein 2 (EBP2), which are nucleolar proteins involved in ribosome biogenesis, and with several ribosomal proteins. NSN1, NSN1-N, and EBP2 co-fractionated primarily with the 60S ribosomal large subunit in vivo. Depletion of NSN1 delayed 25S rRNA maturation and biogenesis of the 60S ribosome subunit, and repressed global translation. NSN1-deficient plants exhibited premature leaf senescence, excessive accumulation of reactive oxygen species, and senescence-related gene expression. Taken together, these results suggest that NSN1 plays a crucial role in plant growth and senescence by modulating ribosome biogenesis.
机译：核糖蛋白是一种核仁GTP结合蛋白，与哺乳动物的干细胞增殖，胚胎发育和核糖体生物发生有关。植物核蛋白样1（NSN1）在胚胎发生，顶端和花分生组织的发展中发挥作用。在这项研究中，确定了核糖体生物发生调控中NSN1的核仁功能。绿色荧光蛋白（GFP）融合的NSN1定位在核仁上，这主要是由其N端结构域决定的。重组NSN1及其N末端域（NSN1-N）在体外与RNA结合。重组NSN1在体外表达GTPase活性。拟南芥和本氏烟草中的NSN1沉默导致生长发育迟缓和衰老。 NSN1与Pescadillo和EBNA1结合蛋白2（EBP2）相互作用，后者是参与核糖体生物发生的核仁蛋白，并与几种核糖体蛋白相互作用。 NSN1，NSN1-N和EBP2在体内主要与60S核糖体大亚基共分离。 NSN1的消耗会延迟25S rRNA的成熟和60S核糖体亚基的生物发生，并抑制整体翻译。缺乏NSN1的植物表现出过早的叶片衰老，活性氧的过度积累以及衰老相关的基因表达。综上所述，这些结果表明NSN1通过调节核糖体的生物发生在植物生长和衰老中起着至关重要的作用。

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