...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Experimental Botany >Transcripts of two ent-copalyl diphosphate synthase genes differentially localize in rice plants according to their distinct biological roles
【24h】

Transcripts of two ent-copalyl diphosphate synthase genes differentially localize in rice plants according to their distinct biological roles

机译：根据其独特的生物学作用，两个对苯二甲酰二磷酸合酶基因的转录本在水稻植株中差异定位

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Gibberellins (GAs) are diterpenoid phytohormones that regulate various aspects of plant growth. Tetracyclic hydrocarbon ent-kaurene is a biosynthetic intermediate of GAs, and is converted from geranylgeranyl diphosphate, a common precursor of diterpenoids, via ent-copalyl diphosphate (ent-CDP) through successive cyclization reactions catalysed by two distinct diterpene synthases, ent-CDP synthase and ent-kaurene synthase. Rice (Oryza sativa L.) has two ent-CDP synthase genes, OsCPS1 and OsCPS2. It has been thought that OsCPS1 participates in GA biosynthesis, while OsCPS2 participates in the biosynthesis of phytoalexins, phytocassanes A-E, and oryzalexins A-F. It has been shown previously that loss-of-function OsCPS1 mutants display a severe dwarf phenotype caused by GA deficiency despite possessing another ent-CDP synthase gene, OsCPS2. Here, experiments were performed to account for the non-redundant biological function of OsCPS1 and OsCPS2. Quantitative reverse transcription-PCR (qRT-PCR) analysis showed that OsCPS2 transcript levels were drastically lower than those of OsCPS1 in the basal parts, including the meristem of the second-leaf sheaths of rice seedlings. qRT-PCR results using tissue samples prepared by laser microdissection suggested that OsCPS1 transcripts mainly localized in vascular bundle tissues, similar to Arabidopsis CPS, which is responsible for GA biosynthesis, whereas OsCPS2 transcripts mainly localized in epidermal cells that address environmental stressors such as pathogen attack. Furthermore, the OsCPS2 transgene under regulation of the OsCPS1 promoter complemented the dwarf phenotype of an OsCPS1 mutant, oscps1-1. The results indicate that transcripts of the two ent-CDP synthase genes differentially localize in rice plants according to their distinct biological roles, OsCPS1 for growth and OsCPS2 for defence.
机译：赤霉素（GAs）是调节植物生长各个方面的二萜类植物激素。四环碳氢化合物正丁香烯是GA的生物合成中间体，并通过两个不同的二萜合酶催化的连续环化反应，通过二磷酸对苯二酚酯（ent-CDP）从对二萜类化合物的常见前体-香叶基香叶基二磷酸酯中转化而来。和ent-kaurene合酶。水稻（Oryza sativa L.）具有两个ent-CDP合酶基因OsCPS1和OsCPS2。已经认为OsCPS1参与了GA的生物合成，而OsCPS2参与了植物抗毒素，植物酪蛋白A-E和稻za毒素A-F的生物合成。先前已证明，功能丧失的OsCPS1突变体尽管具有另一个ent-CDP合酶基因OsCPS2，却显示出由GA缺乏引起的严重矮表型。在这里，进行实验以说明OsCPS1和OsCPS2的非冗余生物学功能。定量逆转录-PCR（qRT-PCR）分析表明，OsCPS2转录本水平显着低于基础部分的OsCPS1转录本，包括水稻幼苗第二叶鞘的分生组织。使用通过激光显微切割制备的组织样品进行的qRT-PCR结果表明，OsCPS1转录本主要位于维管束组织中，类似于拟南芥CPS，后者负责GA生物合成，而OsCPS2转录本主要位于表皮细胞中，这些细胞可以应对环境胁迫（例如病原体攻击） 。此外，在OsCPS1启动子调控下的OsCPS2转基因补充了OsCPS1突变体oscps1-1的矮表型。结果表明，两个ent-CDP合酶基因的转录本根据其独特的生物学作用（用于生长的OsCPS1和用于防御的OsCPS2）差异地定位在水稻植株中。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号