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首页> 外文期刊>Journal of Experimental Botany >Rice putative methyltransferase gene OsTSD2 is required for root development involving pectin modification
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Rice putative methyltransferase gene OsTSD2 is required for root development involving pectin modification

机译：水稻假定的甲基转移酶基因OsTSD2是涉及果胶修饰的根发育所必需的

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摘要

The rice putative methyltransferase gene OsTSD2 is functionally identified and is shown to control root development in a way that involves pectin synthesis and modification in a zone-dependent manner.Pectin synthesis and modification are vital for plant development, although the underlying mechanisms are still not well understood. Here, we report the functional characterization of the OsTSD2 gene, which encodes a putative methyltransferase in rice. All three independent T-DNA insertion lines of OsTSD2 displayed dwarf phenotypes and serial alterations in different zones of the root. These alterations included abnormal cellular adhesion and schizogenous aerenchyma formation in the meristematic zone, inhibited root elongation in the elongation zone, and higher lateral root density in the mature zone. Immunofluorescence (with LM19) and Ruthenium Red staining of the roots showed that unesterified homogalacturonan (HG) was increased in Ostsd2 mutants. Biochemical analysis of cell wall pectin polysaccharides revealed that both the monosaccharide composition and the uronic acid content were decreased in Ostsd2 mutants. Increased endogenous ABA content and opposite roles performed by ABA and IAA in regulating cellular adhesion in the Ostsd2 mutants suggested that OsTSD2 is required for root development in rice through a pathway involving pectin synthesis/modification. A hypothesis to explain the relationship among OsTSD2, pectin methylesterification, and root development is proposed, based on pectin's function in regional cell extension/division in a zone-dependent manner.
机译：水稻推定的甲基转移酶基因OsTSD2在功能上得到了鉴定，并显示出以控制果胶合成和修饰的方式控制根系发育（果胶依赖于区域的方式）。果胶的合成和修饰对于植物发育至关重要，尽管其潜在机制仍不完善了解。在这里，我们报告OsTSD2基因的功能表征，该基因编码水稻中的假定甲基转移酶。 OsTSD2的所有三个独立的T-DNA插入线在根的不同区域均显示出矮表型和序列变化。这些改变包括在分生区异常的细胞粘附和精神分裂性气管形成，在延伸区抑制根伸长以及在成熟区较高的侧根密度。根的免疫荧光（使用LM19）和钌红染色显示，Ostsd2突变体中未酯化的同型半乳糖醛酸（HG）含量增加。细胞壁果胶多糖的生化分析表明，Ostsd2突变体中单糖组成和糖醛酸含量均降低。内源ABA含量的增加以及ABA和IAA在调节Ostsd2突变体中细胞黏附中所起的相反作用表明，OsTSD2是水稻中通过果胶合成/修饰途径进行根发育所必需的。基于果胶在区域细胞扩展/分裂中以区域依赖性方式发挥作用，提出了一种假设来解释OsTSD2，果胶甲基酯化和根发育之间的关系。

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