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首页> 外文期刊>Journal of Experimental Botany >Dissecting stimulus-specific Ca2+ signals in amyloplasts and chloroplasts of Arabidopsis thaliana cell suspension cultures
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Dissecting stimulus-specific Ca2+ signals in amyloplasts and chloroplasts of Arabidopsis thaliana cell suspension cultures

机译：解剖拟南芥细胞悬浮培养的淀粉体和叶绿体中的刺激特异性Ca 2+信号

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Arabidopsis suspension-cultured cells stably expressing aequorin in the plastid stroma were used as a suitable tool to dissect differential Ca2+ responses of non-green plastids versus chloroplasts to several environmental cues.Calcium is used by plants as an intracellular messenger in the detection of and response to a plethora of environmental stimuli and contributes to a fine-tuned internal regulation. Interest in the role of different subcellular compartments in Ca2+ homeostasis and signalling has been growing in recent years. This work has evaluated the potential participation of non-green plastids and chloroplasts in the plant Ca2+ signalling network using heterotrophic and autotrophic cell suspension cultures from Arabidopsis thaliana plant lines stably expressing the bioluminescent Ca2+ reporter aequorin targeted to the plastid stroma. Our results indicate that both amyloplasts and chloroplasts are involved in transient Ca2+ increases in the plastid stroma induced by several environmental stimuli, suggesting that these two functional types of plastids are endowed with similar mechanisms for handling Ca2+. A comparison of the Ca2+ trace kinetics recorded in parallel in the plastid stroma, the surface of the outer membrane of the plastid envelope, and the cytosol indicated that plastids play an essential role in switching off different cytosolic Ca2+ signals. Interestingly, a transient stromal Ca2+ signal in response to the light-to-dark transition was observed in chloroplasts, but not amyloplasts. Moreover, significant differences in the amplitude of specific plastidial Ca2+ changes emerged when the photosynthetic metabolism of chloroplasts was reactivated by light. In summary, our work highlights differences between non-green plastids and chloroplasts in terms of Ca2+ dynamics in response to environmental stimuli.
机译：在质体基质中稳定表达水母发光蛋白的拟南芥悬浮培养细胞被用作分析非绿色质体与叶绿体对几种环境线索的Ca2 +差异反应的合适工具。钙被植物用作细胞内信使，用于检测和应答过多的环境刺激，并有助于微调内部监管。近年来，人们对不同亚细胞区隔在Ca2 +稳态和信号传导中的作用的兴趣不断增长。这项工作已经评估了非绿色质体和叶绿体在植物Ca2 +信号传递网络中的潜在参与程度，使用了来自拟南芥植物系的异养和自养细胞悬浮培养物，稳定表达了针对质体基质的生物发光Ca2 +报告基因水母发光蛋白。我们的结果表明，淀粉质体和叶绿体都参与由几种环境刺激引起的质体基质中Ca2 +的瞬时增加，这表明质体的这两种功能类型具有类似的处理Ca2 +的机制。在质体基质，质体包膜外膜表面和胞质溶胶中平行记录的Ca2 +痕量动力学的比较表明，质体在关闭不同的胞质Ca2 +信号中起重要作用。有趣的是，在叶绿体中观察到响应于明暗转换的瞬时基质Ca2 +信号，但在淀粉体中则没有。此外，当光激活叶绿体的光合代谢时，特定质体Ca 2+变化幅度的显着差异出现。总之，我们的工作着眼于非绿色质体和叶绿体之间在响应环境刺激的Ca2 +动力学方面的差异。

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