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首页> 外文期刊>Journal of Experimental Botany >Extracellular ATP-induced NO production and its dependence on membrane Capo flux in Salvia miltiorrhiza hairy roots.
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Extracellular ATP-induced NO production and its dependence on membrane Capo flux in Salvia miltiorrhiza hairy roots.

机译：丹参毛状根中胞外ATP诱导的NO产生及其对膜变调夹的依赖性。

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摘要

Extracellular ATP (eATP) is a novel signalling agent, and nitric oxide (NO) is a well-established signal molecule with diverse functions in plant growth and development. This study characterizes NO production induced by exogenous ATP and examines its relationship with other important signalling agents, Capo and HO in Salvia miltiorrhiza hairy root culture. Exogenous ATP was applied at 10-500 oM to the hairy root cultures and stimulated NO production was detectable within 30 min. The NO level increased with ATP dose from 10-100 oM but decreased from 100-200 oM or higher. The ATP-induced NO production was mimicked by a non-hydrolysable ATP analogue ATPdS, but only weakly by ADP, AMP or adenosine. The ATP-induced NO production was blocked by Capo antagonists, but not affected by a protein kinase inhibitor. ATP also induced HO production, which was dependent on both Capo and protein kinases, and also on NO biosynthesis. On the other hand, ATP induced a rapid increase in the intracellular Capo level, which was dependent on NO but not HO. The results suggest that NO is implicated in ATP-induced responses and signal transduction in plant cells, and ATP signalling is closely related to Capo and ROS signalling.
机译：细胞外ATP（eATP）是一种新型的信号传导剂，而一氧化氮（NO）是一种成熟的信号分子，在植物生长和发育中具有多种功能。这项研究表征了外源ATP诱导的NO生成，并研究了其与丹参毛状根培养物中其他重要信号传导因子Capo和HO的关系。将外源ATP以10-500 oM的浓度施用于毛状根培养物，并在30分钟内检测到刺激的NO产生。 NO水平随ATP剂量的增加从10-100 oM升高，但从100-200 oM或更高降低。 ATP诱导的NO生成被不可水解的ATP类似物ATPdS模仿，但仅被ADP，AMP或腺苷微弱地模仿。 ATP诱导的NO生成被Capo拮抗剂阻断，但不受蛋白激酶抑制剂的影响。 ATP还诱导HO生成，HO生成依赖于Capo和蛋白激酶，还取决于NO的生物合成。另一方面，ATP诱导细胞内Capo水平快速升高，这取决于NO但不取决于HO。结果表明，NO与植物细胞中ATP诱导的反应和信号转导有关，并且ATP信号与Capo和ROS信号密切相关。

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