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首页> 外文期刊>Journal of Experimental Botany >Functional inactivation of UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) induces early leaf senescence and defence responses in rice
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Functional inactivation of UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) induces early leaf senescence and defence responses in rice

机译：UDP-N-乙酰氨基葡糖焦磷酸化酶1（UAP1）的功能失活诱导水稻早期叶片衰老和防御反应

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摘要

Plant leaf senescence and defence responses are important biological processes, but the molecular mechanisms involved are not well understood. This study identified a new rice mutant, spotted leaf 29 (spl29). The SPL29 gene was identified by map-based cloning, and SPL29 was confirmed as UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) by enzymatic analysis. The mutant spl29 lacks UAP activity. The biological phenotypes for which UAP is responsible have not previously been reported in plants. The spl29 mutant displayed early leaf senescence, confirmed by chlorophyll loss and photosystem II decline as physiological indicators, chloroplast degradation as a cellular characteristic, and both upregulation of senescence transcription factors and senescence-associated genes, and downregulation of photosynthesis-related genes, as molecular evidence. Defence responses were induced in the spl29 mutant, shown by enhanced resistance to bacterial blight inoculation and upregulation of defence response genes. Reactive oxygen species, including O-2(-) and H2O2, accumulated in spl29 plants; there was also increased malondialdehyde content. Enhanced superoxide dismutase activity combined with normal catalase activity in spl29 could be responsible for H2O2 accumulation. The plant hormones jasmonic acid and abscisic acid also accumulated in spl29 plants. ROS and plant hormones probably play important roles in early leaf senescence and defence responses in the spl29 mutant. Based on these findings, it is suggested that UAP1 is involved in regulating leaf senescence and defence responses in rice.
机译：植物叶片的衰老和防御反应是重要的生物学过程，但所涉及的分子机制尚不清楚。这项研究确定了一个新的水稻突变体，斑点叶29（spl29）。通过基于图谱的克隆鉴定出SPL29基因，并通过酶分析证实SPL29为UDP-N-乙酰氨基葡糖焦磷酸化酶1（UAP1）。突变体spl29缺乏UAP活性。 UAP负责的生物表型以前尚未在植物中报道。 spl29突变体显示出早期的叶片衰老，这被叶绿素丢失和光系统II下降作为生理指标，叶绿体降解作为细胞特征以及衰老转录因子和衰老相关基因的上调以及光合作用相关基因的下调（如分子）所证实。证据。在spl29突变体中诱导了防御反应，表现为对细菌疫病接种的抗性增强和防御反应基因的上调。 spl29植物中积累的活性氧，包括O-2（-）和H2O2;丙二醛含量也增加了。增强的超氧化物歧化酶活性与spl29中的正常过氧化氢酶活性相结合可能是H2O2积累的原因。植物激素茉莉酸和脱落酸也积累在spl29植物中。 ROS和植物激素可能在spl29突变体的早期叶片衰老和防御反应中起重要作用。基于这些发现，表明UAP1参与调节水稻的叶片衰老和防御反应。

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