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首页> 外文期刊>Journal of Experimental Botany >PtrABF of Poncirus trifoliata functions in dehydration tolerance by reducing stomatal density and maintaining reactive oxygen species homeostasis
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PtrABF of Poncirus trifoliata functions in dehydration tolerance by reducing stomatal density and maintaining reactive oxygen species homeostasis

机译：Poncirus trifoliata的PtrABF通过降低气孔密度和维持活性氧稳态来发挥脱水耐受性

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摘要

PtrABF, a positive regulator of dehydration tolerance, is involved in stomatal development and regulates polyamine biosynthesis.Abscisic acid-responsive element (ABRE)-binding factors (ABFs) play important roles in abiotic stress responses; however, the underlying mechanisms are poorly understood. In this study, it is reported that overexpression of Poncirus trifoliata PtrABF significantly enhanced dehydration tolerance. The transgenic lines displayed smaller stomatal apertures, reduced stomatal density/index, and lower expression levels of genes associated with stomatal development. PtrABF was found to interact with PtrICE1, a homologue of ICE1 (Inducer of CBF Expression 1) that has been shown to be critical for stomatal development. Microarray analysis revealed that a total of 70 genes were differentially expressed in the transgenic line, 42 induced and 28 repressed. At least two units of ABREs and coupling elements were present in the promoters of most of the induced genes, among which peroxidase and arginine decarboxylase were verified as bona fide targets of PtrABF. Transgenic plants exhibited higher antioxidant enzyme activities and free polyamine levels, but lower levels of reactive oxygen species (ROS) and malondialdehyde. Polyamines were revealed to be associated with ROS scavenging in the transgenic plants due to a modulation of antioxidant enzymes triggered by signalling mediated by H2O2 derived from polyamine oxidase (PAO)-mediated catabolism. Taken together, the results indicate that PtrABF functions positively in dehydration tolerance by limiting water loss through its influence on stomatal movement or formation and maintaining ROS homeostasis via modulation of antioxidant enzymes and polyamines through transcriptional regulation of relevant target genes.
机译：PtrABF是脱水耐受性的正调节剂，参与气孔发育并调节多胺的生物合成。脱落酸反应元件（ABRE）结合因子（ABFs）在非生物胁迫响应中起重要作用;但是，对潜在的机制了解甚少。在这项研究中，据报道三叶草PtrAB的过表达显着增强了脱水耐受性。转基因品系显示较小的气孔孔径，降低的气孔密度/指数以及与气孔发育相关的基因的较低表达水平。发现PtrABF与PtrICE1相互作用，PtrICE1是ICE1（CBF表达1的诱导物）的同源物，已被证明对气孔发育至关重要。基因芯片分析显示，在转基因品系中共有70个基因差异表达，其中42个基因被诱导，而28个基因被抑制。大多数诱导基因的启动子中存在至少两个单位的ABRE和偶联元件，其中过氧化物酶和精氨酸脱羧酶被证实是PtrABF的真正靶标。转基因植物表现出较高的抗氧化酶活性和游离多胺水平，但较低的活性氧（ROS）和丙二醛水平。由于多胺氧化酶（PAO）介导的分解代谢中的H2O2介导的信号触发了抗氧化酶的调节，因此发现多胺与转基因植物中的ROS清除有关。两者合计，结果表明，PtrABF通过限制水分损失（通过其对气孔运动或形成的影响）和通过调节抗氧化剂酶和多胺的转录调控相关目标基因来维持ROS稳态，从而在脱水耐受中发挥积极作用。

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