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首页> 外文期刊>Journal of Experimental Botany >Expression of a RING-HC protein from rice improves resistance to Pseudomonas syringae pv. tomato DC3000 in transgenic Arabidopsis thaliana
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Expression of a RING-HC protein from rice improves resistance to Pseudomonas syringae pv. tomato DC3000 in transgenic Arabidopsis thaliana

机译：从水稻中表达RING-HC蛋白可提高对丁香假单胞菌pv的抗性。转基因拟南芥中的番茄DC3000

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摘要

A cDNA clone (OsRHC1) was obtained, which encodes a novel RING zinc finger protein sharing similar structural features (multiple transmembrane domains at the N-half; a unique RING zinc finger consensus Cys-X(2)-Cys-X(11)-Cys-X-His-X(3)-Cys-X(2)-Cys-X(6)-Cys-X(2)-Cys at the C terminus) to a group of closely related annotated proteins from both monocots and dicots. OsRHC1 was found to be localized on plasma membrane of rice cells and induced by wounding in rice lines containing Xa loci. Ecotopic expression of the OsRHC1 cDNA from rice (a monocot) in transgenic Arabidopsis thaliana (a dicot) enhanced the defence response toward Pseudomonas syringae pv. tomato DC3000, suggesting that OsRHC1 may confer broad-spectrum disease resistance. The protective effects were neutralized in the presence of MG132 or in an npr1-3 mutation background, indicating that the function of OsRHC1 is dependent on the ubiquitin-mediated protein degradation via the 26S proteasome and the presence of the key defence response regulator NPR1.
机译：获得了一个cDNA克隆（OsRHC1），该cDNA编码一种新型RING锌指蛋白，该蛋白具有相似的结构特征（在N半处具有多个跨膜结构域;一个独特的RING锌指共识Cys-X（2）-Cys-X（11） -Cys-X-His-X（3）-Cys-X（2）-Cys-X（6）-Cys-X（2）-Cys在C末端）到一组来自两个单子叶植物的密切相关的带注释的蛋白质和双子叶植物。发现OsRHC1位于水稻细胞的质膜上，并且是通过在含有Xa基因座的水稻系中受伤而诱导的。水稻（单子叶植物）中的OsRHC1 cDNA在转基因拟南芥（双子叶植物）中的异位表达增强了对丁香假单胞菌pv的防御反应。番茄DC3000，表明OsRHC1可能赋予广谱抗病性。在存在MG132或npr1-3突变背景下，保护作用被中和，表明OsRHC1的功能取决于通过26S蛋白酶体的泛素介导的蛋白质降解以及关键防御反应调节剂NPR1的存在。

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