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首页> 外文期刊>Journal of Experimental Botany >Identification and characterization of an expansin gene AsEXP1 associated with heat tolerance in C3 Agrostis grass species.
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Identification and characterization of an expansin gene AsEXP1 associated with heat tolerance in C3 Agrostis grass species.

机译：C 3 Agrostis草种中耐热性相关的扩展蛋白AsEXP1的鉴定和表征。

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摘要

Plant tolerance of heat stress involves various changes at physiological and molecular levels. The objective of this study was to examine the expression of a gene encoding expansin protein in relation to heat tolerance in two C< sub>3 grass species and genotypes differing in heat tolerance. Heat-tolerant, thermal Agrostis scabra, adapted to high temperatures in geothermal areas in Yellowstone National Park, was subjected to 20 degrees C (control) or 40 degrees C (heat stress) for 7 d in a growth chamber. Differential display analysis identified that a gene, AsEXP1, encoding an expansin protein, was strongly up-regulated in leaves exposed to heat stress in thermal A. scabra. Virtual northern hybridization and RT-PCR confirmed that AsEXP1 was a heat-inducible gene in leaves. The expression of AsEXP1 was induced at 1 h of plant exposure to heat stress and reached the highest level of expression at 4 h of treatment. A 1.3 kb full-length cDNA of AsEXP1 was isolated, which encodes a 251 amino acid protein. Two ecotypes of thermal A. scabra and 10 genotypes of Agrostis stolonifera (creeping bentgrass), a widely used turfgrass species in cool climatic regions, varying in the level of heat tolerance, were exposed to 40 degrees C for 7 d to examine the level of AsEXP1 expression in relation to heat tolerance. Genetic variation in heat tolerance was evaluated by measuring cell membrane stability, photochemical efficiency, and leaf growth. RT-PCR analysis revealed that the level of AsEXP1 in different genotypes was positively correlated with the level of heat tolerance in both grass species. The results first identified a heat-related expansin gene in grass species and suggest that AsEXP1 may be useful as a molecular marker to select for heat-tolerant grass germplasm.
机译：植物对热胁迫的耐受性涉及生理和分子水平的各种变化。这项研究的目的是要检查在两个C 3 草种和耐热性不同的基因型中，与热耐受性相关的编码扩展蛋白的基因的表达。将耐热的Agrostis scabra耐热材料适应黄石国家公园地热地区的高温，在生长室中使其经受20摄氏度（对照）或40摄氏度（热应力）的作用7 d。差异显示分析确定，编码expansin蛋白的基因AsEXP1在暴露于高温thermal草热胁迫的叶片中强烈上调。虚拟Northern杂交和RT-PCR证实AsEXP1是叶片中的热诱导基因。 AsEXP1的表达在植物暴露于热胁迫1 h时被诱导，并在处理4 h达到最高表达水平。分离出一个1.3 kb的AsEXP1全长cDNA，它编码251个氨基酸。将两种生态型的regions麦草和10个基因型的ros草（cre本草）（一种在寒冷气候区域广泛使用的草皮物种）在不同的耐热水平下暴露于40°C下7天，以检查AsEXP1表达与耐热性有关。通过测量细胞膜稳定性，光化学效率和叶片生长来评估耐热性的遗传变异。 RT-PCR分析表明，两种基因型中不同基因型的AsEXP1水平与耐热性水平呈正相关。结果首先在草种中鉴定了与热相关的expanin基因，并表明AsEXP1可用作分子标记来选择耐热草种质。

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