...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Experimental Botany >Variation in genomic methylation in natural populations of Populus simonii is associated with leaf shape and photosynthetic traits
【24h】

Variation in genomic methylation in natural populations of Populus simonii is associated with leaf shape and photosynthetic traits

机译：小叶杨自然种群中基因组甲基化的变化与叶片形状和光合特性有关

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

DNA methylation, one of the best-studied types of chromatin modification, suppresses the expression of transposable elements, pseudogenes, repetitive sequences, and individual genes. However, the extent and variation of genome-wide DNA methylation in natural populations of plants remain relatively unknown. To investigate variation in DNA methylation and whether this variation associates with important plant traits, including leaf shape and photosynthesis, 20 413 DNA methylation sites were examined in a poplar association population (505 individuals) using methylation-sensitive amplification polymorphism (MSAP) technology. Calculation of epi-population structure and kinships assigned individuals into subsets (K=3), revealing that the natural population of P. simonii consists of three subpopulations. Population epigenetic distance and geographic distance showed a significant correlation (r=0.4688, P<0.001), suggesting that environmental factors may affect epigenetics. Single-marker approaches were also used to identify significant marker-trait associations, which found 1087 high-confidence DNA methylation markers associated with different phenotypic traits explaining similar to 5-15% of the phenotypic variance. Among these loci, 147 differentially methylated fragments were obtained by sequencing, representing 130 candidate genes. Expression analysis of six candidate genes indicated that these genes might play important roles in leaf development and regulation of photosynthesis. This study provides association analysis to study the effects of DNA methylation on plant development and these data indicate that epigenetics bridges environmental and genetic factors in affecting plant growth and development.
机译：DNA甲基化是研究最深入的染色质修饰类型之一，可抑制转座因子，假基因，重复序列和单个基因的表达。但是，植物的自然种群中全基因组DNA甲基化的程度和变异仍然相对未知。为了研究DNA甲基化的变异以及这种变异是否与重要的植物性状（包括叶片形状和光合作用）相关联，使用甲基化敏感性扩增多态性（MSAP）技术在杨树协会种群（505个个体）中检查了20 413个DNA甲基化位点。计算上位种群结构和亲属关系将个体分为亚组（K = 3），这表明西蒙氏疟原虫的自然种群由三个亚种群组成。种群表观遗传距离与地理距离呈显着相关性（r = 0.4688，P <0.001），表明环境因素可能影响表观遗传。单标记方法还用于识别显着的标记-性状关联，发现了1087个与不同表型性状相关的高可信度DNA甲基化标记，解释了约5-15％的表型变异。在这些基因座中，通过测序获得了147个差异甲基化片段，代表130个候选基因。对六个候选基因的表达分析表明，这些基因可能在叶片发育和光合作用的调节中起重要作用。这项研究提供关联分析，以研究DNA甲基化对植物发育的影响，这些数据表明表观遗传学在影响植物生长和发育的环境和遗传因素之间架起了桥梁。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号