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首页> 外文期刊>Journal of Experimental Botany >Differential expression of GS5 regulates grain size in rice
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Differential expression of GS5 regulates grain size in rice

机译：GS5的差异表达调节水稻的籽粒大小

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Two SNPs in the promoter of GS5 are responsible for expression variation controlling grain size. Enhanced expression of GS5 competitively inhibits the interaction between OsBAK1 and OsMSBP1, promoting grain size.Grain weight is a major determinant of grain yield. GS5 is a positive regulator of grain size such that grain width, filling, and weight are correlated with its expression level. Previous work suggested that polymorphisms of GS5 in the promoter region might be responsible for the variation in grain size. In this study, two single nucleotide polymorphisms (SNPs) between the wide-grain allele GS5-1 and the narrow-grain allele GS5-2 in the upstream region of the gene that were responsible for the differential expression in developing young panicles were identified. These two polymorphs altered the responses of the GS5 alleles to abscisic acid (ABA) treatments, resulting in higher expression of GS5-1 than of GS5-2 in developing young panicles. It was also shown that SNPs in light-responsive elements of the promoter altered the response to light induction, leading to higher expression of GS5-2 than GS5-1 in leaves. Enhanced expression of GS5 competitively inhibits the interaction between OsBAK1-7 and OsMSBP1 by occupying the extracellular leucine-rich repeat (LRR) domain of OsBAK1-7, thus preventing OsBAK1-7 from endocytosis caused by interacting with OsMSBP1, providing an explanation for the positive association between grain size and GS5 expression. These results advanced our understanding of the molecular mechanism by which GS5 controls grain size.
机译：GS5启动子中的两个SNP负责控制晶粒大小的表达变化。 GS5的增强表达竞争性地抑制了OsBAK1和OsMSBP1之间的相互作用，从而促进了晶粒大小。粒重是决定谷物产量的主要因素。 GS5是晶粒尺寸的正调节剂，因此晶粒宽度，填充度和重量与其表达水平相关。先前的工作表明，启动子区域中GS5的多态性可能是晶粒尺寸变化的原因。在这项研究中，确定了该基因上游区域中的宽粒等位基因GS5-1和窄粒等位基因GS5-2之间的两个单核苷酸多态性（SNP），它们负责发育中的幼穗的差异表达。这两个多态性改变了GS5等位基因对脱落酸（ABA）处理的反应，导致发育中的幼穗中GS5-1的表达高于GS5-2的表达。还显示，启动子的光响应元件中的SNP改变了对光诱导的响应，从而导致GS5-2在叶片中的表达高于GS5-1。 GS5的增强表达通过占据OsBAK1-7的富含细胞亮氨酸的重复序列（LRR）域来竞争性抑制OsBAK1-7和OsMSBP1之间的相互作用，从而防止OsBAK1-7与OsMSBP1相互作用引起的内吞作用，为阳性表达提供了解释大小和GS5表达之间的关联。这些结果提高了我们对GS5控制晶粒尺寸的分子机理的理解。

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