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首页> 外文期刊>Journal of Experimental Botany >Rice nitrate transporter OsNPF2.4 functions in low-affinity acquisition and long-distance transport
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Rice nitrate transporter OsNPF2.4 functions in low-affinity acquisition and long-distance transport

机译：水稻硝酸盐转运蛋白OsNPF2.4在低亲和力获取和长距离运输中起作用

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摘要

Plant proteins belonging to the NPF (formerly NRT1/PTR) family are well represented in every genome and function in transporting a wide variety of substrates. In this study, we showed that rice OsNPF2.4 is located in the plasma membrane and is expressed mainly in the epidermis, xylem parenchyma, and phloem companion cells. Functional analysis in oocytes showed that OsNPF2.4 is a pH-dependent, low-affinity NO3- transporter. Short-term (NO3-)-N-15 influx rate, long-term NO3- acquisition by root, and upward transfer from root to shoot were decreased by disruption of OsNPF2.4 and increased by OsNPF2.4 overexpression under high NO3- supply. Moreover, the redistribution of NO3- in the mutants in comparison with the wild type from the oldest leaf to other organs, particularly to N-starved roots, was dramatically changed. Knockout of OsNPF2.4 decreased rice growth and potassium (K) concentration in xylem sap, root, culm, and sheath, but increased the shoot: root ratio of tissue K under higher NO3-. We conclude that OsNPF2.4 functions in acquisition and long-distance transport of NO3-, and that altering its expression has an indirect effect on K recycling between the root and shoot.
机译：属于NPF（以前称为NRT1 / PTR）家族的植物蛋白在每个基因组中都有很好的代表，并在运输各种底物方面发挥作用。在这项研究中，我们表明水稻OsNPF2.4位于质膜中，并主要在表皮，木质部薄壁组织和韧皮部伴随细胞中表达。卵母细胞的功能分析表明OsNPF2.4是pH依赖性的低亲和力NO3转运蛋白。在高NO3供应下，OsNPF2.4的破坏减少了短期（NO3-）-N-15的流入速度，根系对NO3的长期吸收以及从根到根的向上转移，而OsNPF2.4的过表达则增加了从根向芽的向上转移。 。此外，与野生型相比，从最老的叶子​​到其他器官，特别是到N饥饿的根，野生型中NO3-的重新分布发生了巨大变化。 OsNPF2.4的敲除降低了水稻生长和木质部汁液，根，茎和鞘中钾（K）的浓度，但在较高的NO3-下增加了组织K的地上部：根部比率。我们得出的结论是，OsNPF2.4在NO3-的获取和远距离运输中起作用，并且改变其表达对根与梢之间的K循环有间接影响。

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