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首页> 外文期刊>Journal of Experimental Botany >Cytochrome P450 CYP71BE5 in grapevine (Vitis vinifera) catalyzes the formation of the spicy aroma compound (-)-rotundone
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Cytochrome P450 CYP71BE5 in grapevine (Vitis vinifera) catalyzes the formation of the spicy aroma compound (-)-rotundone

机译：葡萄（Vitis vinifera）中的细胞色素P450 CYP71BE5催化形成辛辣香气化合物（-）-酮

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摘要

(-)-Rotundone is a potent odorant molecule with a characteristic spicy aroma existing in various plants including grapevines (Vitis vinifera). It is considered to be a significant compound in wines and grapes because of its low sensory threshold and aroma properties. (-)-Rotundone was first identified in red wine made from the grape cultivar Syrah and here we report the identification of VvSTO2 as a alpha-guaiene 2-oxidase which can transform alpha-guaiene to (-)-rotundone in the grape cultivar Syrah. It is a cytochrome P450 (CYP) enzyme belonging to the CYP 71BE subfamily, which overlaps with the very large CYP71D family and, to the best of our knowledge, this is the first functional characterization of an enzyme from this family. VvSTO2 was expressed at a higher level in the Syrah grape exocarp (skin) in accord with the localization of (-)-rotundone accumulation in grape berries. alpha-Guaiene was also detected in the Syrah grape exocarp at an extremely high concentration. These findings suggest that (-)-rotundone accumulation is regulated by the VvSTO2 expression along with the availability of a-guaiene as a precursor. VvSTO2 expression during grape maturation was considerably higher in Syrah grape exocarp compared to Merlot grape exocarp, consistent with the patterns of a-guaiene and (-)-rotundone accumulation. On the basis of these findings, we propose that VvSTO2 may be a key enzyme in the biosynthesis of (-)-rotundone in grapevines by acting as a alpha-guaiene 2-oxidase.
机译：（-）-Rotundone是一种强力的气味分子，具有特征性的辛辣香气，存在于各种植物中，包括葡萄树（Vitis vinifera）。由于其低的感官阈值和香气特性，它被认为是葡萄酒和葡萄中的重要化合物。 （-）-Rotundone最早是在用葡萄栽培品种Syrah酿造的红酒中鉴定的，在这里我们报道了VvSTO2作为α-番石榴烯2-氧化酶的鉴定，该酶可以将α-番石榴烯转化为Syrah葡萄栽培品种。它是一种属于CYP 71BE亚家族的细胞色素P450（CYP）酶，与非常大的CYP71D家族重叠，据我们所知，这是该家族酶的第一个功能表征。 VySTO2在西拉（Syrah）葡萄外果皮中的表达水平较高，这与葡萄浆果中（-）-酮的积累位置一致。在西拉葡萄外果皮中也发现了非常高浓度的α-瓜烯。这些发现表明（-）-酮的积累受VvSTO2表达以及α-愈创木烯作为前体的可用性的调节。与美乐葡萄外果皮相比，西拉葡萄外果皮中VvSTO2的表达要高得多，这与α-番石榴烯和（-）-雄酮积累的模式一致。基于这些发现，我们提出VvSTO2可能是葡萄中（-）-酮的生物合成中的关键酶，它可以充当α-番石榴烯2-氧化酶。

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