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首页> 外文期刊>Journal of Experimental Botany >The cotton MYB108 forms a positive feedback regulation loop with CML11 and participates in the defense response against Verticillium dahliae infection
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The cotton MYB108 forms a positive feedback regulation loop with CML11 and participates in the defense response against Verticillium dahliae infection

机译：棉花MYB108与CML11形成正反馈调节环，并参与针对大黄萎病菌的防御反应

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Accumulating evidence indicates that plant MYB transcription factors participate in defense against pathogen attack, but their regulatory targets and related signaling processes remain largely unknown. Here, we identified a defense-related MYB gene (GhMYB108) from upland cotton (Gossypium hirsutum) and characterized its functional mechanism. Expression of GhMYB108 in cotton plants was induced by Verticillium dahliae infection and responded to the application of defense signaling molecules, including salicylic acid, jasmonic acid, and ethylene. Knockdown of GhMYB108 expression led to increased susceptibility of cotton plants to V. dahliae, while ecotopic overexpression of GhMYB108 in Arabidopsis thaliana conferred enhanced tolerance to the pathogen. Further analysis demonstrated that GhMYB108 interacted with the calmodulin-like protein GhCML11, and the two proteins form a positive feedback loop to enhance the transcription of GhCML11 in a calcium-dependent manner. Verticillium dahliae infection stimulated Ca2+ influx into the cytosol in cotton root cells, but this response was disrupted in both GhCML11-silenced plants and GhMYB108-silenced plants in which expression of several calcium signaling-related genes was down-regulated. Taken together, these results indicate that GhMYB108 acts as a positive regulator in defense against V. dahliae infection by interacting with GhCML11. Furthermore, the data also revealed the important roles and synergetic regulation of MYB transcription factor, Ca2+, and calmodulin in plant immune responses.
机译：越来越多的证据表明，植物MYB转录因子参与了抗病原体攻击的防御，但其调控靶标和相关信号传导过程仍然未知。在这里，我们从陆地棉（陆地棉）中鉴定了一个与防御相关的MYB基因（GhMYB108），并对其功能机制进行了描述。黄萎病菌感染可引起棉花植物中GhMYB108的表达，并响应防御信号分子（包括水杨酸，茉莉酸和乙烯）的应用。击倒GhMYB108表达导致棉花植物对大麦弧菌的敏感性增加，而拟南芥中GhMYB108的生态位过表达赋予对病原体的耐受性增强。进一步的分析表明，GhMYB108与钙调蛋白样蛋白GhCML11相互作用，并且这两种蛋白形成正反馈环，以钙依赖性方式增强GhCML11的转录。黄萎病菌的感染刺激了Ca2 +流入棉花根细胞的胞质溶胶中，但这种反应在GhCML11沉默的植物和GhMYB108沉默的植物中均受到干扰，这些植物中一些钙信号相关基因的表达被下调。综上所述，这些结果表明，GhMYB108通过与GhCML11相互作用，在防御大丽弧菌感染中起着积极的调节作用。此外，数据还揭示了MYB转录因子，Ca2 +和钙调蛋白在植物免疫应答中的重要作用和协同调节。

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