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首页> 外文期刊>Journal of Experimental Botany >Plastidic phosphoglucomutase and ADP-glucose pyrophosphorylase mutants impair starch synthesis in rice pollen grains and cause male sterility
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Plastidic phosphoglucomutase and ADP-glucose pyrophosphorylase mutants impair starch synthesis in rice pollen grains and cause male sterility

机译：质体磷酸葡萄糖变位酶和ADP-葡萄糖焦磷酸化酶突变体会破坏水稻花粉粒中的淀粉合成并导致雄性不育

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摘要

Analysis of rice plastidic phosphoglucomutase and ADP-glucose pyrophosphorylase mutants reveals that starch synthesis in pollen grains requires the production of glucose-1-P and ADP-glucose in the plastids.To elucidate the starch synthesis pathway and the role of this reserve in rice pollen, we characterized mutations in the plastidic phosphoglucomutase, OspPGM, and the plastidic large subunit of ADP-glucose (ADP-Glc) pyrophosphorylase, OsAGPL4. Both genes were up-regulated in maturing pollen, a stage when starch begins to accumulate. Progeny analysis of self-pollinated heterozygous lines carrying the OspPGM mutant alleles, osppgm-1 and osppgm-2, or the OsAGPL4 mutant allele, osagpl4-1, as well as reciprocal crosses between the wild type (WT) and heterozygotes revealed that loss of OspPGM or OsAGPL4 caused male sterility, with the former condition rescued by the introduction of the WT OspPGM gene. While iodine staining and transmission electron microscopy analyses of pollen grains from homozygous osppgm-1 lines produced by anther culture confirmed the starch null phenotype, pollen from homozygous osagpl4 mutant lines, osagpl4-2 and osagpl4-3, generated by the CRISPR/Cas system, accumulated small amounts of starch which were sufficient to produce viable seed. Such osagpl4 mutant pollen, however, was unable to compete against WT pollen successfully, validating the important role of this reserve in fertilization. Our results demonstrate that starch is mainly polymerized from ADP-Glc synthesized from plastidic hexose phosphates in rice pollen and that starch is an essential requirement for successful fertilization in rice.
机译：水稻质体磷酸葡萄糖磷酸变位酶和ADP-葡萄糖焦磷酸化酶突变体的分析表明，花粉粒中的淀粉合成需要在质体中产生葡萄糖-1-P和ADP-葡萄糖，以阐明淀粉合成途径及其在水稻花粉中的作用。 ，我们表征了质体磷酸葡萄糖突变酶OspPGM和ADP-葡萄糖（ADP-Glc）焦磷酸化酶OsAGPL4的质体大亚基中的突变。在成熟的花粉中，这两个基因均被上调，这是淀粉开始积累的阶段。对携带OspPGM突变体等位基因osppgm-1和osppgm-2或OsAGPL4突变体等位基因osagpl4-1的自花授粉杂合系进行后代分析，以及野生型（WT）和杂合子之间的相互杂交表明OspPGM或OsAGPL4引起了雄性不育，通过导入WT OspPGM基因挽救了前者的状况。尽管通过花药培养产生的纯合osppgm-1品系的花粉粒的碘染色和透射电镜分析证实了CRISPR / Cas系统产生的淀粉无效表型，纯合osagpl4突变体品系osagpl4-2和osagpl4-3的花粉，积累了少量的淀粉，足以产生有活力的种子。但是，这种osagp14突变体花粉不能成功地与野生型花粉竞争，从而证实了该储备在受精中的重要作用。我们的结果表明淀粉主要是由水稻花粉中质性己糖磷酸酯合成的ADP-Glc聚合而成的，淀粉是水稻成功受精的基本要求。

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