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首页> 外文期刊>Journal of Experimental Botany >CaLecRK-S.5, a pepper L-type lectin receptor kinase gene, confers broad-spectrum resistance by activating priming
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CaLecRK-S.5, a pepper L-type lectin receptor kinase gene, confers broad-spectrum resistance by activating priming

机译：CaLecRK-S.5是胡椒L型凝集素受体激酶基因，通过激活引物赋予广谱抗性

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In Arabidopsis, several L-type lectin receptor kinases (LecRKs) have been identified as putative immune receptors. However, to date, there have been few analyses of LecRKs in crop plants. Virus-induced gene silencing of CaLecRK-S.5 verified the role of CaLecRK-S.5 in broad-spectrum resistance. Compared with control plants, CaLecRK-S.5-silenced plants showed reduced hypersensitive response, reactive oxygen species burst, secondary metabolite production, mitogen-activated protein kinase activation, and defense-related gene expression in response to Tobacco mosaic virus pathotype P-0 (TMV-P-0) infection. Suppression of CaLecRK-S.5 expression significantly enhanced the susceptibility to Pepper mild mottle virus pathotype P-1,P-2,P-3, Xanthomonas campestris pv. vesicatoria, Phytophthora capsici, as well as TMV-P-0. Additionally, beta-aminobutyric acid treatment and a systemic acquired resistance assay revealed that CaLecRK-S.5 is involved in priming of plant immunity. Pre-treatment with beta-aminobutyric acid before viral infection restored the reduced disease resistance phenotypes shown in CaLecRK-S.5-silenced plants. Systemic acquired resistance was also abolished in CaLecRK-S.5-silenced plants. Finally, RNA sequencing analysis indicated that CaLecRK-S.5 positively regulates plant immunity at the transcriptional level. Altogether, these results suggest that CaLecRK-S.5-mediated broad-spectrum resistance is associated with the regulation of priming.
机译：在拟南芥中，几种L型凝集素受体激酶（LecRKs）已被确定为推定的免疫受体。但是，迄今为止，对农作物中LecRK的分析很少。病毒诱导的CaLecRK-S.5基因沉默证实了CaLecRK-S.5在广谱抗性中的作用。与对照植物相比，CaLecRK-S.5沉默的植物显示对烟草花叶病毒病态P-0响应的过敏反应减少，活性氧爆发，次级代谢产物产生，丝裂原活化的蛋白激酶活化和防御相关基因表达（TMV-P-0）感染。 CaLecRK-S.5表达的抑制显着增强了对胡椒轻斑驳病毒致病型P-1，P-2，P-3，油菜黄单胞菌pv的敏感性。 vesicatoria，疫霉菌（Phytophthora capsici）以及TMV-P-0。此外，β-氨基丁酸处理和系统获得性抗药性测定表明，CaLecRK-S.5与植物免疫力的启动有关。在病毒感染之前用β-氨基丁酸进行预处理可以恢复CaLecRK-S.5沉默植物中表现出的降低的抗病性表型。在CaLecRK-S.5沉默的植物中，系统性获得性抗性也被消除。最后，RNA测序分析表明CaLecRK-S.5在转录水平上正调控植物的免疫力。总之，这些结果表明，CaLecRK-S.5介导的广谱抗性与启动调控有关。

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