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首页> 外文期刊>Journal of Equine Veterinary Science >Validation of the Lactate Plus Lactate Meter in the Horse and Its Use in a Conditioning Program
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Validation of the Lactate Plus Lactate Meter in the Horse and Its Use in a Conditioning Program

机译：马匹中乳酸加乳酸计的验证及其在调节程序中的使用

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摘要

The equine industry has a need for a convenient, rapid, and reliable method of measuring blood lactate concentrations ([LA]). We hypothesized that the handheld Lactate Plus lactate meter (LPlus), developed and tested for use in humans, would provide dependable results when used in horses undergoing an exercise conditioning program and that horse's fitness would improve following individualized conditioning based on each horse's velocity at which [LA] = 4 mmol/L (V-LA4) was reached. Five adult horses underwent a 4-week training program that consisted of 3 exercise bouts/wk. Horses were subjected to an incremental step standardized exercise test (SET) before starting (SET-1) and after the completion of the program (SET-2). Blood samples were collected before each increase in speed until [LA] reached >= 4 mmol/L, and then the SET was terminated. The [LA] sample range in our study was 0-8 mmol/L. Blood was analyzed at the time of collection using a calibrated LPlus, and plasma was collected for [LA] determination using the lactate dehydrogenase-based enzymatic colorimetric method. Although the LPlus tended to significantly underestimate [LA] by 0.39 mmol/L (P .001), the LPlus proved to be a dependable device for use in horses based on good correlation with the biochemical analysis (r = 0.978) and Bland-Altman limits of agreement and 95% confidence intervals. All horses showed an increase in V-LA4 from SET-1 to SET-2, consistent with improved fitness following our 3 exercise bout/wk training protocol. The LPlus can reliably be used in horses to determine [LA] ranging from 0-8 mmol/L. When determining serial [LA], analytical techniques should not be used interchangeably. (C) 2014 Elsevier Inc. All rights reserved.
机译：马工业需要一种方便，快速，可靠的测量血液乳酸浓度（[LA]）的方法。我们假设开发并测试用于人的手持式乳酸加乳酸计（LPlus），在用于进行运动调节程序的马匹中使用时，将提供可靠的结果，并且根据每匹马的速度进行个性化调节后，马匹的适应性将会提高[LA]达到4 mmol / L（V-LA4）。五匹成年马接受了为期4周的训练，每星期3次。在开始（SET-1）之前和完成程序（SET-2）之后，对马进行增量式步长标准化运动测试（SET）。在每次速度增加之前收集血样，直到[LA]达到> = 4 mmol / L，然后终止SET。我们研究中的[LA]样品范围为0-8 mmol / L。在收集时使用校准的LPlus分析血液，并使用基于乳酸脱氢酶的酶比色法收集血浆用于[LA]测定。尽管LPlus倾向于将[LA]大大低估0.39 mmol / L（P <.001），但基于与生化分析的良好相关性（r = 0.978）和Bland-奥特曼协议的限制和95％的置信区间。所有马匹都显示出从SET-1到SET-2的V-LA4升高，这与我们进行3次运动训练/周训练方案后健身的改善一致。 LPlus可以可靠地用于马匹中，测定[LA]的范围为0-8 mmol / L。确定序列[LA]时，分析技术不应互换使用。 （C）2014 Elsevier Inc.保留所有权利。

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