...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of feline medicine and surgery >Development of monoclonal antibodies (MAbs) to feline interferon (fIFN)- gamma as tools to evaluate cellular immune responses to feline infectious peritonitis virus (FIPV).
【24h】

Development of monoclonal antibodies (MAbs) to feline interferon (fIFN)- gamma as tools to evaluate cellular immune responses to feline infectious peritonitis virus (FIPV).

机译：针对猫干扰素（fIFN）-γ的单克隆抗体（MAb）的开发作为评估对猫传染性腹膜炎病毒（FIPV）的细胞免疫反应的工具。

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Feline infectious peritonitis virus (FIPV) can cause a lethal disease in cats, feline infectious peritonitis (FIP). The antibody-dependent enhancement (ADE) of FIPV infection has been recognised in experimentally infected cats, and cellular immunity is considered to play an important role in preventing the onset of FIP. To evaluate the importance of cellular immunity for FIPV infection, monoclonal antibodies (MAbs) against feline interferon (fIFN)- gamma were first created to establish fIFN- gamma detection systems using the MAbs. Six anti-fIFN- gamma MAbs were created. Then, the difference in epitope which those MAbs recognise was demonstrated by competitive enzyme-linked immunosorbent assay (ELISA) and IFN- gamma neutralisation tests. Detection systems for fIFN- gamma (sandwich ELISA, ELISpot assay, and two-colour flow cytometry) were established using anti-fIFN- gamma MAbs that recognise different epitopes. In all tests, fIFN- gamma production from peripheral blood mononuclear cells (PBMCs) obtained from cats experimentally infected with an FIPV isolate that did not develop the disease was significantly increased by heat-inactivated FIPV stimulation in comparison with medium alone. Especially, CD8+fIFN- gamma + cells, but not CD4+fIFN- gamma + cells, were increased. In contrast, fIFN- gamma production from PBMCs isolated from cats that had developed FIP and specific pathogen-free (SPF) cats was not increased by heat-inactivated FIPV stimulation. These results suggest that cellular immunity plays an important role in preventing the development of FIP. Measurement of fIFN- gamma production with the anti-fIFN- gamma MAbs created in this study appeared to be useful in evaluating cellular immunity in cats
机译：猫传染性腹膜炎病毒（FIPV）可以在猫中引起致命性疾病，猫传染性腹膜炎（FIP）。 FIPV感染的抗体依赖性增强（ADE）在实验感染的猫中已得到公认，并且细胞免疫被认为在预防FIP发作中起重要作用。为了评估细胞免疫对FIPV感染的重要性，首先创建了针对猫干扰素（fIFN）-γ的单克隆抗体（MAb），以建立使用MAb的fIFN-γ检测系统。创建了六种抗fIFN-γMAb。然后，通过竞争性酶联免疫吸附测定（ELISA）和IFN-γ中和测试证明了那些MAb识别的表位的差异。使用识别不同表位的抗fIFN-γMAb，建立了fIFN-γ的检测系统（夹心ELISA，ELISpot分析和双色流式细胞术）。在所有测试中，与单独的培养基相比，通过热灭活的FIPV刺激显着增加了实验性感染FIPV分离株的猫的外周血单核细胞（PBMC）产生的fIFN-γ量，而该FIPV分离株并未发展成该病。特别是，CD8 + fIFN-γ + 细胞增加了，但CD4 + fIFN-γ + 细胞没有增加。 。相比之下，热灭活的FIPV刺激不会增加从已发展为FIP的猫和特定的无病原体（SPF）猫中分离出的PBMC产生的fIFN-γ。这些结果表明细胞免疫在防止FIP的发展中起重要作用。用这项研究中创建的抗fIFN-γMAb来测量fIFN-γ的产生似乎可用于评估猫的细胞免疫力

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号