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首页> 外文期刊>Journal of Fish Biology >Characterization of a novel cell line from the caudal fin of koi carp Cyprinus carpio
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Characterization of a novel cell line from the caudal fin of koi carp Cyprinus carpio

机译：锦鲤鲤尾鳍新细胞系的表征

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摘要

A continuous cell line (KF-101) derived from the caudal fin of the koi carp Cyprinus carpio was established and characterized. The KF-101 cell line multiplied abundantly in Leibovitz's L-15 medium containing 10% foetal bovine serum at 25 degrees C, and was subcultured for >90 passages over a period of 3years. Immunocytochemistry revealed that the KF-101 cells contain keratin, junction proteins connexin-43 and occludin, and ectodermal stem-cell marker Pax-6, but not vimentin. Furthermore, the KF-101 cells reacted with anti-human DARPP-32 and anti-human GATA-4 antibodies, and the labelling was regulated according to the cell cycle. The labels of the DARPP-32 and GATA-4 antibodies in the KF-101 cells were the suggested phosphatase-1 inhibitor-1 and GATA-3, respectively. In addition, the KF-101 cells were susceptible to koi herpesvirus but were resistant to eel herpesvirus, iridovirus, grouper nodavirus and chum salmon (Oncorhynchus keta) virus. The results indicate that the KF-101 cells are suitable materials for investigating biological and virological development.
机译：建立并表征了来自锦鲤鲤尾鳍的连续细胞系（KF-101）。 KF-101细胞系在含有10％胎牛血清的Leibovitz's L-15培养基中在25摄氏度下大量繁殖，并在3年​​的时间内传代> 90代。免疫细胞化学显示，KF-101细胞含有角蛋白，连接蛋白connexin-43和occludin，以及外胚层干细胞标记Pax-6，但不含波形蛋白。此外，KF-101细胞与抗人DARPP-32和抗人GATA-4抗体发生反应，并根据细胞周期调节标记。 KF-101细胞中DARPP-32和GATA-4抗体的标记分别是建议的磷酸酶-1抑制剂-1和GATA-3。此外，KF-101细胞对锦鲤疱疹病毒敏感，但对鳗疱疹病毒，虹膜病毒，石斑鱼诺达病毒和Chum鲑（Oncorhynchus keta）病毒有抗性。结果表明，KF-101细胞是用于研究生物学和病毒学发展的合适材料。

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