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首页> 外文期刊>Journal of Fish Biology >Trypsin and chymotrypsin as indicators of nutritional status of post-weaned sea bass larvae.
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Trypsin and chymotrypsin as indicators of nutritional status of post-weaned sea bass larvae.

机译：胰蛋白酶和胰凝乳蛋白酶作为断奶后鲈鱼幼虫营养状况的指标。

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摘要

The aim of the present work was to evaluate to what extent variation in the activity of either trypsin or chymotrypsin could be a sensitive and reliable indicator of nutritional deficiencies during post-weaning stages in a marine fish. Sea bass Dicentrarchus labrax larvae obtained from two different spawns classified as high quality (HQ) or low quality (LQ) on the basis of their hatching variables, were used to evaluate if enzyme production could be conditioned by factors other than those related to the feeding regime. In addition, larvae in each group were either fed normally or received a ration reduced by 60%. Trypsin and chymotrypsin activities were separately measured in larvae at days 3 and 15 after weaning. Changes in mass and final survival were also recorded. Food limitation resulted in a lower final mass only in the HQ larvae. Survival was significantly higher in HQ larvae, but also in larvae suffering food restriction, irrespective of the quality of the spawn. In both groups of larvae, lower food availability was correlated to increased values of trypsin, and particularly, of chymotrypsin. Variations were high enough to support the usefulness of such enzymes as indicators of a modified nutritional status. It is suggested that the noticeable increase in the activity of both enzymes observed in the restrictedly fed larvae could compensate initial deficiencies and explain the good results in growth and survival obtained in LQ larvae..
机译：本工作的目的是评估海鱼断奶后阶段胰蛋白酶或胰凝乳蛋白酶活性的变化在多大程度上可以作为营养不足的敏感而可靠的指标。从其孵化变量分为高品质（HQ）或低品质（LQ）的两个不同产卵中获得的鲈鱼鲈鱼幼虫，用于评估酶的产生是否可以由与饲喂相关的因素以外的因素来调节政权。此外，每组的幼虫都可以正常喂食或减少60％的日粮。在断奶后第3和15天，分别在幼虫中测量胰蛋白酶和胰凝乳蛋白酶的活性。还记录了质量变化和最终生存率。食物的限制仅在总部幼虫中导致较低的最终质量。无论产卵质量如何，总部幼虫的存活率均显着较高，但受食物限制的幼虫的存活率也较高。在两组幼虫中，较低的食物利用率与胰蛋白酶，尤其是胰凝乳蛋白酶的值升高相关。变异很高，足以支持这些酶作为营养状况改善指标的有用性。提示在限制性饲喂的幼虫中观察到的两种酶活性的显着增加可以弥补最初的缺陷，并可以解释LQ幼虫在生长和存活方面取得的良好结果。

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