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首页> 外文期刊>Journal of Fish Biology >Olfactory sensitivity to conspecific bile fluid and skin mucus in the European eel Anguilla anguilla (L.
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Olfactory sensitivity to conspecific bile fluid and skin mucus in the European eel Anguilla anguilla (L.

机译:嗅觉对欧洲鳗鳗的特异性胆汁液和皮肤粘液的敏感性(L.

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摘要

The present study assessed the olfactory potency of conspecific bile fluid and skin mucus in the European eel Anguilla anguilla by the electro-olfactogram. Immature males showed high olfactory sensitivity to conspecific bile, giving large amplitude responses in a concentration-dependent manner with estimated thresholds of detection of <1:10 super(7) (n= 6). Mucus also proved to contain highly potent odorants with thresholds of detection of c. 1:10 super(6) (n= 6). Crude solid-phase extraction of bile fluid (C-18 and C-2/ENV+ cartridges) showed that the majority of olfactory activity in bile fluid was contained in the eluate of C-18 cartridges (n= 6). There were quantitative differences, however, between the sexes; female bile fluid had a higher proportion of activity in this fraction. Similar solid-phase extraction of mucus showed that it contains a higher proportion of odorants in the C-18 filtrate than bile fluid. Mucus from mature eels, however, had a higher proportion of olfactory activity in the eluate than immature fish (n= 6). Cross-adaptation experiments suggest that there are qualitative differences in the odorants contained in bile and mucus depending on both the sex and state of sexual maturation of the donor (n= 6). These results are consistent with a role for chemical communication in the reproduction of the European eel and suggest that both bile and mucus are potential sources of the odorants involved.
机译:本研究通过电-嗅觉图评估了欧洲鳗gu中的特异性胆汁液和皮肤粘液的嗅觉效能。未成熟的雄性对同种胆汁表现出较高的嗅觉敏感性,以浓度依赖的方式提供大幅度的响应,估计的检测阈值<1:10 super(7)(n = 6)。粘液还被证明含有强效的气味物质,其检测阈值为c。 1:10超级(6)(n = 6)。胆汁(C-18和C-2 / ENV +药筒)的粗固相萃取显示,胆汁中的大部分嗅觉活性都包含在C-18药筒的洗脱液中(n = 6)。但是,性别之间存在数量差异。雌性胆汁液在该部分中具有较高比例的活性。相似的固相粘液萃取表明,C-18滤液中的胆味剂含量比胆汁液高。然而,来自成熟鳗鱼的粘液在洗脱液中的嗅觉活性比未成熟鱼高(n = 6)。交叉适应实验表明,胆汁和粘液中所含的气味物质在质量上存在差异,这取决于供体的性别和性成熟状态(n = 6)。这些结果与化学通讯在欧洲鳗鱼繁殖中的作用是一致的,并且表明胆汁和粘液都是所涉及的增香剂的潜在来源。

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