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首页> 外文期刊>Journal of Fish Biology >The efficacy of field techniques for obtaining and storing blood samples from fishes.
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The efficacy of field techniques for obtaining and storing blood samples from fishes.

机译：现场技术从鱼类中获取和储存血液样本的功效。

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Prompted by the dramatic increase in the use of blood analyses in fisheries research and monitoring, this study investigated the efficacy of common field techniques for sampling and storing blood from fishes. Three questions were addressed: (1) Do blood samples taken via rapid caudal puncture (the 'grab-and-stab' technique) yield similar results for live v. sacrificed groups of fishes? (2) Do rapidly obtained caudal blood samples accurately represent blood properties of fishes prior to capture? (3) Does storage of whole blood in an ice slurry for a working day (8.5 h) modify the properties of the plasma? It was shown that haematocrit, plasma ions, metabolites, stress hormones and sex hormones of caudal blood samples were statistically similar when taken from live v. recently sacrificed groups of adult coho salmon Oncorhynchus kisutch. Moreover, this study confirmed by using paired blood samples from cannulated O. kisutch that blood acquired through the caudal puncture technique (mean+or-S.E. 142+or-26 s after capture) was representative of fish prior to capture. Long-term (8.5 h) cold storage of sockeye salmon Oncorhynchus nerka whole blood caused significant decreases in plasma potassium and chloride, and a significant increase in plasma glucose. Previous research has suggested that these changes largely result from net movements of ions and molecules between the plasma and erythrocytes, movements that can occur within minutes of storage. Thus, blood samples from fishes should be centrifuged as quickly as practicable in the field for separation of plasma and erythrocytes to prevent potentially misleading data.
机译：在渔业研究和监测中大量使用血液分析的推动下，本研究调查了用于采样和储存鱼类血液的通用现场技术的功效。解决了三个问题：（1）通过快速尾椎穿刺（'grab-and-stab'技术）采集的血液样本对活着的 v。牺牲组产生了相似的结果鱼？ （2）快速获得的尾部血样是否能准确地代表捕获前鱼类的血液特性？ （3）将全血保存在冰浆中一个工作日（8.5小时）是否会改变血浆的特性？结果表明，从活体 v 中采集的尾血样本的血细胞比容，血浆离子，代谢产物，应激激素和性激素在统计学上是相似的。我>。而且，该研究通过使用来自空心iO的成对的血液样本得到证实。 kisutch 通过尾部穿刺技术获得的血液（捕获后平均+或-S.E. 142+或-26 s）代表了捕获前的鱼。红鲑鱼 Oncorhynchus nerka 全血的长期（8.5小时）冷藏会导致血浆钾和氯的显着减少，以及血浆葡萄糖的显着增加。先前的研究表明，这些变化很大程度上是由于离子和分子在血浆和红细胞之间的净运动所引起的，这种运动可能在储存的几分钟内发生。因此，鱼类的血样应在现场尽可能快地离心，以分离血浆和红细胞，以防止潜在的误导性数据。

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