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首页> 外文期刊>Journal of Fish Diseases >Sequence variation in the genes encoding the major capsid protein, the ATPase, and the RNA polymerase 2 (domain 6) of lymphocystis disease virus isolated from Schlegel's black rockfish, Sebastes schlegelii Hilgendorf.
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Sequence variation in the genes encoding the major capsid protein, the ATPase, and the RNA polymerase 2 (domain 6) of lymphocystis disease virus isolated from Schlegel's black rockfish, Sebastes schlegelii Hilgendorf.

机译：分离自Schlegel的黑石鱼Sebastes schlegelii Hilgendorf的淋巴囊病病毒的主要衣壳蛋白，ATPase和RNA聚合酶2（结构域6）的基因中的序列变异。

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The aim of this study was to investigate the genetic characteristics of lymphocystis disease virus (LCDV) isolates from diseased Schlegel's black rockfish Sebastes schlegelii. Viral DNA was extracted from the skin lesions of rockfish and the major capsid protein (MCP) gene, adenosine triphosphatase (ATPase) gene and RNA polymerase 2 (domain 6) (Rpb2-6) gene were located, sequenced and compared with homologous genes from other LCDVs. DNA sequencing was performed using an automated DNA sequencer. Sequence analyses of the full-length ORFs of the three genes revealed the presence of genomic variants among LCDV isolates. Although one of the MCP gene sequences from rockfish was highly homologous (99%), the others from the flounder showed low sequence identity (80-85%) with the sequence data from the rockfish. The ATPase gene and the Rpb2-6 gene sequences from rockfish also showed low sequence identity (70-84%) with those from flounders. The MCP protein sequence from rockfish showed relatively high sequence identity (87-98%) to other LCDV isolates. However, there was only 71-86% identity in the ATPase protein and 77-88% in the Rpb2-6 protein. The findings revealed that LDCVs from rockfish showed low identities with those from flounder. The sequences of the MCP, ATPase and Rpb2-6 genes between rockfish and flounder were identical and strain variants existed. Sequence data reported in this paper have been deposited in GenBank under accession numbers AY823414 (MCP), AY947406 (ATPase) and DQ004274 (Rpb2-6)..
机译：这项研究的目的是调查患病的Schlegel的黑石鱼Sebastes schlegelii的淋巴囊病病毒（LCDV）分离株的遗传特征。从石鱼的皮肤损伤中提取病毒DNA，并定位主要衣壳蛋白（MCP）基因，腺苷三磷酸酶（ATPase）基因和RNA聚合酶2（结构域6）（Rpb2-6）基因，对其进行测序，并与该基因的同源基因进行比较。其他LCDV。使用自动DNA测序仪进行DNA测序。对这三个基因的全长ORF进行序列分析，发现LCDV分离株中存在基因组变异。尽管来自石鱼的MCP基因序列之一具有高度同源性（99％），但来自比目鱼的其他MCP基因序列与石鱼的序列数据显示出较低的序列同一性（80-85％）。岩鱼的ATPase基因和Rpb2-6基因序列与比目鱼的序列同一性较低（70-84％）。岩鱼的MCP蛋白序列与其他LCDV分离株具有较高的序列同一性（87-98％）。但是，ATPase蛋白中只有71-86％的同一性，Rpb2-6蛋白中只有77-88％的同一性。研究结果表明，石鱼的LDCV与比目鱼的LDCV的同源性较低。石鱼和比目鱼之间的MCP，ATPase和Rpb2-6基因的序列相同，并且存在菌株变异。本文报道的序列数据已以登录号AY823414（MCP），AY947406（ATPase）和DQ004274（Rpb2-6）保存在GenBank中。

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