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首页> 外文期刊>Journal of Fish Diseases >Atypical bacterial gill disease: a new form of bacterial gill disease affecting intensively reared salmonids
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Atypical bacterial gill disease: a new form of bacterial gill disease affecting intensively reared salmonids

机译:非典型细菌性disease病:一种影响密集饲养鲑鱼的新型细菌性disease病

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An unusual form of bacterial gill disease (BGD) was identified affecting 5 species of cultured salmonids from Canada (rainbow trout, chinook salmon and Atlantic salmon), Norway (brown trout) and Chile (coho salmon). All outbreaks occurred at low water temperatures (<10deg C) and with clinical presentations distinct from classical BGD, which is caused by Flavobacterium branchiophilum. Mortality of affected fish increased, but it was not as precipitous as in classical BGD. Histopathology revealed largenumbers of small bacterial rods adhering to the lamellar epithelium of affected gills from all outbreaks. The lamellar epithelium appeared swollen, often with evidence of single cell degeneration and exfoliation. In more severe instances, the formation of lamellar synechiae was seen, usually associated with sequestration of bacteria between fused lamellae. Overt epithelial hyperplasia, lamellar fusion and filamental clubbing were not common sequelae to infection; instead, the end result was shortened and somewhat stubby lamellae covered with swollen epithelial cells. The predominant bacterium recovered from affected gills was a small, Gram-negative, motile, fluorescent pigment-producing rod that shared phenotypic characteristics with Pseudomonas fluorescens. Polyclonal antisera prepared against 3 representative isolates indicated a weak antigenic similarity among them. Immunohistochemistry corroborated this finding. A Gram-negative, yellow pigmented bacterium (YPB), identified as Flavobacterium psychrophilum, was also recovered, but only from the gills in the Ontario outbreaks. Antiserum prepared against this YPB indicated antigenic similarity among isolates recovered from the Ontario outbreaks, but immunohistochemistry failed to recognize antigenically related bacteria on the gills of fish from the other outbreaks. Based on the unusual clinical presentation and the histopathological appearance of the gills this condition was termed 'atypical bacterial gill disease' (ABGD).
机译:确定了一种不寻常的细菌性g病(BGD),影响了来自加拿大(虹鳟鱼,奇努克鲑鱼和大西洋鲑鱼),挪威(褐鳟)和智利(银大麻哈鱼)的5种养殖鲑鱼。所有暴发都发生在低水温(<10℃)下,并且临床表现与经典BGD不同,经典BGD是由分支杆菌黄杆菌引起的。受影响鱼类的死亡率增加了,但不像传统的BGD那样严重。组织病理学显示,在所有暴发中,大量小细菌棒附着在受影响腮的片状上皮上。层状上皮似乎肿胀,通常有单细胞变性和脱落的迹象。在更严重的情况下,可以看到层状粘连的形成,通常与细菌在融合层之间的隔离有关。明显的上皮上皮增生,层状融合和丝状棍打不常见。取而代之的是,缩短了最终结果,并在表面上铺上了肿胀的上皮细胞。从受影响的ill中回收的主要细菌是小的革兰氏阴性,能动的荧光色素产生棒,与荧光假单胞菌具有表型特征。针对3个代表性分离株制备的多克隆抗血清表明它们之间的抗原相似性较弱。免疫组织化学证实了这一发现。还发现了革兰氏阴性,黄色素细菌(YPB),被鉴定为精神黄杆菌(Flavobacterium psychrophilum),但仅从安大略省暴发的the中回收。针对这种YPB制备的抗血清表明,从安大略省暴发中分离出的分离株之间存在抗原相似性,但免疫组织化学未能识别其他暴发中的鱼the上的抗原相关细菌。基于the的异常临床表现和组织病理学表现,这种情况被称为“非典型细菌性disease病”(ABGD)。

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