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首页> 外文期刊>Journal of Fish Diseases >Lymphoid organ cell culture system from Penaeus monodon (Fabricius) as aTI Lymphoid organ cell culture system from Penaeus monodon (Fabricius) as a platform for white spot syndrome virus and shrimp immune-related gene expression
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Lymphoid organ cell culture system from Penaeus monodon (Fabricius) as aTI Lymphoid organ cell culture system from Penaeus monodon (Fabricius) as a platform for white spot syndrome virus and shrimp immune-related gene expression

机译：斑节对虾（Fabricius）的淋巴器官细胞培养系统作为aTI斑节对虾（Fabricius）的淋巴器官细胞培养系统作为白斑综合症病毒和虾免疫相关基因表达的平台

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Shrimp cell lines are yet to be reported and this restricts the prospects of investigating the associated viral pathogens, especially white spot syndrome virus (WSSV). In this context, development of primary cell cultures from lymphoid organs was standardized. Poly-l-lysine-coated culture vessels enhanced growth of lymphoid cells, while the application of vertebrate growth factors did not, except insulin-like growth factor-1 (IGF-1). Susceptibility of the lymphoid cells to WSSV was confirmed by immunofluoresence assay using monoclonal antibody against the 28 kDa envelope protein of WSSV. Expression of viral and immune-related genes in WSSV-infected lymphoid cultures could be demonstrated by RT-PCR. This emphasizes the utility of lymphoid primary cell culture as a platform for research in viruscell interaction, virus morphogenesis, up and downregulation of shrimp immune-related genes, and also for the discovery of novel drugs to combat WSSV in shrimp culture.
机译：虾细胞系尚未报道，这限制了研究相关病毒病原体，特别是白斑综合症病毒（WSSV）的前景。在这种情况下，来自淋巴器官的原代细胞培养的发展已标准化。涂有聚赖氨酸的培养皿可促进淋巴样细胞的生长，而脊椎动物生长因子的应用则没有，除了胰岛素样生长因子-1（IGF-1）。使用针对WSSV的28 kDa包膜蛋白的单克隆抗体，通过免疫荧光分析证实了淋巴样细胞对WSSV的敏感性。病毒和免疫相关基因在WSSV感染的淋巴样培养物中的表达可通过RT-PCR证明。这强调了淋巴样原代细胞培养作为研究病毒细胞相互作用，病毒形态发生，对虾免疫相关基因的上调和下调以及研究对虾培养物中抗WSSV的新药的平台的实用性。

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