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首页> 外文期刊>Journal of Fish Biology >Resolution of the Acanthopagrus black seabream complex based on mitochondrial and amplified fragment-length polymorphism analyses.
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Resolution of the Acanthopagrus black seabream complex based on mitochondrial and amplified fragment-length polymorphism analyses.

机译:基于线粒体和扩增的片段长度多态性分析解决棘棘黑鲷的复杂问题。

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摘要

In this study, DNA analyses were employed to verify the identity of six morphologically similar species that occur in the coastal waters of Taiwan: the black seabream complex (Acanthopagrus latus, Acanthopagrus schlegelii, Acanthopagrus sivicolus, Acanthopagrus taiwanensis, Acanthopagrus chinshira and Acanthopagrus pacificus). Amplified fragment-length polymorphism (AFLP) analyses clearly distinguished the same six species that are morphologically diagnosable based on subtle differences in scale counts and anal-fin colouration. In contrast, mitochondrial DNA analyses based on cytochrome b gene sequences did not distinguish individuals of A. schlegelii and A. sivicolus, reflecting either historical introgression or recent speciation and incomplete sorting of their mitochondrial lineages. Phylogenetic relationships among these six north-west Pacific Ocean species of Acanthopagrus analysed using AFLP data were consistent with scale rows above the lateral line (TRac), sperm ultrastructure and geographical distribution. The study provides molecular tools for future research relevant to improved management of these resources, and an increased understanding of the evolutionary history of this radiation. Copyright 2011 The Authors. Journal of Fish Biology Copyright 2011 The Fisheries Society of the British Isles.CAS Registry/EC Number/Name of Substance 0 (DNA, Mitochondrial). 9035-37-4 (Cytochromes b).
机译:在这项研究中,通过DNA分析来验证台湾沿海水域中出现的六个形态相似的物种的身份:黑鲷鱼(黑棘鱼,白棘鱼,白棘鱼,台湾白棘鱼,中华白棘鱼​​和白棘鱼)。扩增的片段长度多态性(AFLP)分析清楚地区分了基于形态计数和肛门鳍着色的细微差别在形态学上可诊断的相同六个物种。相比之下,基于细胞色素b基因序列的线粒体DNA分析不能区分schlegelii和A. sivicolus个体,反映了其线粒体谱系的历史渗入或近来物种形成和不完整排序。使用AFLP数据分析的这六个西北太平洋棘皮动物物种之间的亲缘关系与侧线(TRac)上方的鳞片行,精子超微结构和地理分布一致。该研究为未来的研究提供了分子工具,这些工具与改进对这些资源的管理以及对这种辐射的进化历史的了解增加了有关。版权所有2011作者。 《鱼类生物学杂志》,2011年,不列颠群岛渔业协会,CAS注册/ EC编号/物质名称0(DNA,线粒体)。 9035-37-4(细胞色素b)。

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