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首页> 外文期刊>Journal of Fish Diseases >Bacterial enteritis and the development of the larval digestive tract in olive flounder, Paralichthys olivaceus (Temminck & Schlegel)
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Bacterial enteritis and the development of the larval digestive tract in olive flounder, Paralichthys olivaceus (Temminck & Schlegel)

机译：橄榄比目鱼，Paralichthys olivaceus（Temminck＆Schlegel），细菌性肠炎和幼虫消化道的发育

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摘要

Three bacterial isolates obtained from diseased olive flounder larvae, Paralichthys olivaceus, were identified as Vibrio ichthyoenteri based on the results of phenotypic characterization and 16S rRNA gene sequencing studies. Bacterial enteritis was reproduced in 16 and 22 days post-hatch (dph) larvae by administering brine shrimp nauplii, Artemia salina, dosed with the environmental isolates and reference strains of V. ichthyoenteri. To investigate the effect of the disease on development of the stomach, a pepsin activity assay and reverse transcription-polymerase chain reaction (RT-PCR) analysis of the expression of the pepsinogen gene were performed. Expression of olive flounder pepsinogen was detected from 30-dph larvae and the increased level of pepsin activity coincided with reduced susceptibility to the disease. Growth rates of V. ichthyoenteri, V. anguillarum and Edwardsiella tarda were tested in artificial stomach conditions using HCl and porcine pepsin. All the strains of V. ichthyoenteri were inhibited by low pH conditions which corresponded with an increase in pepsin levels. This suggests that differentiation of the stomach in olive flounder larvae and juveniles, an essential physiological development, also provides the host with a non-immunological defence mechanism. [References: 27]
机译：根据表型特征和16S rRNA基因测序研究的结果，从患病的橄榄比目鱼幼虫中分离到的三种细菌被鉴定为伊氏弧菌。在孵化后（dph）的幼虫中，通过施用盐水虾无节幼体，卤虫（Artemia salina），并添加环境分离株和ichthyoenteri参照菌株，可繁殖细菌性肠炎。为了研究该疾病对胃发育的影响，进行了胃蛋白酶活性测定和胃蛋白酶原基因表达的逆转录-聚合酶链反应（RT-PCR）分析。从30 dph幼虫中检测到比目鱼胃蛋白酶原的表达，胃蛋白酶活性的增加与对疾病的易感性降低相吻合。用盐酸和猪胃蛋白酶在人工胃条件下测试了ichthyoenteri，V。anguillarum和tard Edwardsiella的生长速率。低pH条件抑制了ichthyoenteri的所有菌株，这与胃蛋白酶水平的增加相对应。这表明，在比目鱼幼虫和幼鱼中胃的分化是一种重要的生理发育，它也为宿主提供了非免疫防御机制。 [参考：27]

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