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首页> 外文期刊>Journal of Food Measurement and Characterization >In vitro antioxidant, brine shrimp lethality and antimicrobial activities of methanol and petroleum ether extracts of Cassia renigera fruit
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In vitro antioxidant, brine shrimp lethality and antimicrobial activities of methanol and petroleum ether extracts of Cassia renigera fruit

机译：决明子甲醇和石油醚提取物的体外抗氧化剂，卤水虾杀伤力和抗菌活性。

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摘要

Cassia renigera (C. renigera) is an evergreen tree (family Caesalpiniaceae) is locally known as Minjiri (Bengali). It is locally used as a medicinal plant. In this study we investigated antioxidant, brine shrimp lethality and antimicrobial activitiesof the fruit extracts of this plant using different in vitro assay models. Methanol extract showed better activities than petroleum ether extract in total phenol, total flavonoid and total antioxidant capacity assays. Besides, methanol extract also showed higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Nitric oxide radical scavenging activities than petroleum ether extract. In cupric reducing and reducing power capacity assays methanol extract exhibited dose dependent reducing capacity. In brine shrimplethality bioassay petroleum ether extract (LC50 of 89.385 μg/mL, χ2 = 215.968, p < 0.05) was found to be more potent than methanol extract (LC50 of 128.042 μg/mL, χ2 = 235.274, p < 0.05). In disk diffusion technique, it was observed that Gram positive bacteria are more susceptible than Gram negative bacteria. Both extracts showed broad spectrum antimicrobial activity. Methanol extract showed antibacterial activity against all tested microorganisms. The highest activity (minimum zone of inhibition 11.25 ± 0.35 mm) was exhibited by methanol extract against Bacillus cerus. No activity was observed against Escherichia coli and Shigella dysenteriae by petroleum ether extract. In a nutshell, it is suggested that the fruit can be used as a potential source for the above mentioned bioactivities. That is why extensive researches may be carried out to investigate the active principles responsible for these activities.
机译：决明子C. renigera（C. renigera）是一棵常绿的树（家庭Caesalpiniaceae），在当地被称为Minjiri（孟加拉）。在当地被用作药用植物。在这项研究中，我们使用不同的体外测定模型研究了该植物果实提取物的抗氧化剂，盐水虾的致死性和抗菌活性。在总酚，总黄酮和总抗氧化能力的测定中，甲醇提取物显示出比石油醚提取物更好的活性。此外，甲醇提取物还显示出比石油醚提取物更高的1,1-二苯基-2-甲基苯并肼（DPPH）和一氧化氮自由基清除活性。在铜还原和还原能力测定中，甲醇提取物表现出剂量依赖性的还原能力。在卤水虾致命性生物测定中，发现石油醚提取物（LC50为89.385μg/ mL，χ2= 215.968，p <0.05）比甲醇提取物（LC50为128.042μg/ mL，χ2= 235.274，p <0.05）更有效。在盘扩散技术中，观察到革兰氏阳性细菌比革兰氏阴性细菌更易感。两种提取物均具有广谱抗菌活性。甲醇提取物显示出对所有测试微生物的抗菌活性。甲醇提取物对蜡样芽孢杆菌具有最高的活性（最小抑菌圈为11.25±0.35 mm）。石油醚提取物未观察到针对大肠杆菌和痢疾志贺氏菌的活性。简而言之，建议该水果可用作上述生物活性的潜在来源。这就是为什么可能要进行大量研究以调查造成这些活动的积极原则的原因。

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