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首页> 外文期刊>Journal of Food Science and Technology >Optimization of selective production media for enhanced production of xylanases in submerged fermentation by Thielaviopsis basicola MTCC 1467 using L-16 orthogonal array
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Optimization of selective production media for enhanced production of xylanases in submerged fermentation by Thielaviopsis basicola MTCC 1467 using L-16 orthogonal array

机译：使用L-16正交阵列优化选择性发酵培养基，以提高在深层发酵的基础上的拟南芥MTCC 1467发酵中木聚糖酶的产量

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摘要

Enzymes have been the centre of attention for researchers/industrialists worldwide due to their wide range of physiological, analytical, food/feed and industrial based applications. Among the enzymes explored for industrial applications, xylanases play an instrumental role in food/feed, textile/detergent, paper and biorefinery based application sectors. This study deals with the statistical optimization of xylanase production by Thielaviopsis basicola MTCC 1467 under submerged fermentation conditions using rice straw, as sole carbon source. Different fermentation parameters such as carbon source, nitrogen source, inorganic salts like KH2PO4, MgSO4 and pH of the medium were optimized at the individual and interactive level by Taguchi orthogonal array methodology (L-16). All selected fermentation parameters influenced the enzyme production. Rice straw, the major carbon source mainly influenced the production of xylanase (similar to 34 %). After media optimization, the yield of enzyme improved from 38 to similar to 60 IU/ml (161.5 %) indicating the commercial production of xylanase by T. basicola MTCC 1467. This study shows the potential of T. basicola MTCC 1467 for the efficient xylanase production under the optimized set of conditions
机译：酶由于其广泛的生理，分析，食品/饲料和工业应用而成为全球研究人员/工业界关注的焦点。在工业应用中探索的酶中，木聚糖酶在食品/饲料，纺织/洗涤剂，造纸和生物炼制应用领域中发挥着重要作用。这项研究处理在深水发酵条件下，以稻草作为唯一碳源的基础上，Thielaviopsis basicola MTCC 1467对木聚糖酶生产的统计优化。通过Taguchi正交阵列方法（L-16）在单个和交互作用的水平上优化了不同的发酵参数，例如碳源，氮源，无机盐（如KH2PO4，MgSO4和培养基的pH）。所有选定的发酵参数都会影响酶的产生。稻草是主要的碳源，主要影响木聚糖酶的产量（约占34％）。经过培养基优化后，酶的产率从38提高到接近60 IU / ml（161.5％），表明T. basicola MTCC 1467可以商业化生产木聚糖酶。这项研究显示了T. basicola MTCC 1467对于高效木聚糖酶的潜力。在最佳条件下生产

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