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首页> 外文期刊>Journal of general plant pathology >Taxonomic characterization of Pyricularia isolates from green foxtail and giant foxtail, wild foxtails in Japan
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Taxonomic characterization of Pyricularia isolates from green foxtail and giant foxtail, wild foxtails in Japan

机译：日本的绿色狐尾和巨型狐尾，野生狐尾的拟南芥分离株的分类学表征

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摘要

Twenty-eight Pyricularia isolates from two wild foxtails--green foxtail (Setaria viridis) and giant foxtail (S. faberii)--in Japan were taxonomically characterized by DNA analyses, mating tests, and pathogenicity assays. Although most of the isolates failed to produce perithecia in mating tests with Magnaporthe oryzae, a diagnostic polymerase chain reaction-restriction fragment length polymorphism phenotype of M. oryzae was detected in the beta-tubulin genomic region in all isolates. The pathogenicity assays revealed that host ranges of the isolates were similar to those of isolates from foxtail millet (S. italica), which were exclusively pathogenic on foxtail millet. In addition to the 28 isolates from wild foxtails, 22 Pyricularia isolates from 11 other grasses were analyzed by RFLP using single-copy sequences as probes. In a dendrogram constructed from the RFLP data, isolates that were previously identified as M. oryzae formed a single cluster. All the wild foxtail isolates formed a subcluster with foxtail millet isolates within the M. oryzae cluster. From these results, we conclude that Pyricularia isolates from the wild foxtails are closely related to isolates from foxtail millet and should be classified into the Setaria pathotype of M. oryzae.
机译：通过DNA分析，交配试验和致病性分析，对日本的两种野生狐尾的28个拟南芥分离株进行了分类学鉴定，其中绿色狐尾（Setaria viridis）和巨型狐尾（S. faberii）。尽管大多数分离株在与稻瘟病菌的交配试验中未能产生皮膜病，但在所有分离株的β-微管蛋白基因组区域中均检测到了米曲霉的诊断性聚合酶链反应-限制性片段长度多态性表型。致病性分析表明，分离株的寄主范围与谷子（S. italica）的分离株相似，后者仅对谷子有致病性。除了野生狐尾草的28种分离物外，还使用单拷贝序列作为探针，通过RFLP分析了来自11种其他草的22种稻瘟病菌。在根据RFLP数据构建的树状图中，以前被鉴定为米曲霉的分离株形成了单个簇。所有野生的狐尾分离物与米曲霉簇中的谷子分离物形成亚簇。从这些结果，我们得出结论，来自野生狐尾的拟南芥分离株与来自谷子小isolate的分离株密切相关，应归类为米曲霉的狗尾草病菌。

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