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首页> 外文期刊>Journal of genetics >Upregulation of miR-222 in both Helicobacter pylori-infected and noninfected gastric cancer patients
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Upregulation of miR-222 in both Helicobacter pylori-infected and noninfected gastric cancer patients

机译：幽门螺杆菌感染和未感染的胃癌患者中miR-222的上调

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摘要

Despite of promising improvements in treatment of gastric cancer, the mortality rate of this malignancy remains high. Chronic infection by Helicobacter pylori, interfering with intracellular signalling pathways, is the main risk factor for gastric cancer. Some evidence suggests that microRNAs (miRNA), the small noncoding RNA molecules, can play role as oncogenes or tumour suppressors in the cells. MiR-222 is one of the remarkable miRNAs undergoing upregulation in gastric cancer. However, the association between miR-222 upregulation and H. pylori infection in gastric cancer tissues remains unclear. The aim of this study was to analyse the expression level of miR-222 in gastric cancer tissues, evaluating the relationship between miR-222 expression level and H. pylori infection and also finding novel miR-222 targets based on in silico investigations. MiR-222 expression level in 200 patients including 112 H. pylori positive and 88 H. pylori negative was relatively measured using RT-qPCR and compared with 88 healthy samples. In silico enrichment analysis of miR-222 targets was performed by DAVID database to evaluate the possible role(s) of miR-222 in gastric tumourigenesis. We observed upregulated level of miR-222 in gastric cancer tissues compared with normal samples (P<0.05). However, no significant difference between miR-222 expression in H. pylori-positive and H. pylori-negative cases was observed. Our in silico analyses showed the possible role of p53, p27, PTEN and Elongin B in gastric cancer tumourigenesis. MiR-222 functions as an onco-miRNA and its overexpression can be involved in pathogenesis of gastric cancer, independent of H. pylori infection.
机译：尽管在胃癌的治疗方面有望改善，但是这种恶性肿瘤的死亡率仍然很高。幽门螺杆菌的慢性感染，干扰细胞内信号传导途径，是胃癌的主要危险因素。一些证据表明，微小的非编码RNA分子microRNA（miRNA）可以在细胞中充当癌基因或抑癌基因。 MiR-222是在胃癌中经历上调的杰出miRNA之一。然而，miR-222上调与胃癌组织中幽门螺杆菌感染之间的关联仍不清楚。这项研究的目的是分析miR-222在胃癌组织中的表达水平，评估miR-222表达水平与幽门螺杆菌感染之间的关系，并根据计算机研究发现新的miR-222靶标。使用RT-qPCR相对测量了200名患者的MiR-222表达水平，包括112例幽门螺杆菌阳性和88例幽门螺杆菌阴性，并与88例健康样本进行了比较。通过DAVID数据库对miR-222目标进行了计算机富集分析，以评估miR-222在胃肿瘤发生中的可能作用。与正常样品相比，我们观察到胃癌组织中miR-222的水平上调（P <0.05）。但是，在幽门螺杆菌阳性和幽门螺杆菌阴性病例中，miR-222表达之间没有显着差异。我们的计算机分析表明p53，p27，PTEN和Elongin B在胃癌肿瘤发生中的可能作用。 MiR-222充当癌基因miRNA，其过表达可参与胃癌的发病机理，而与幽门螺杆菌感染无关。

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