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首页> 外文期刊>Journal of genetics >Isolation, characterization and mapping of genes differentially expressed during fibre development between Gossypium hirsutum and G. barbadense by cDNA-SRAP
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Isolation, characterization and mapping of genes differentially expressed during fibre development between Gossypium hirsutum and G. barbadense by cDNA-SRAP

机译：陆地棉和巴氏假丝酵母在纤维发育过程中差异表达基因的分离，鉴定和定位

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摘要

Gossypium hirsutum and G. barbadense are two cultivated tetraploid cotton species with differences in fibre quality. The fibre of G. barbadense is longer, stronger and finer than that of G. hirsutum. To isolate genes expressed differently between the two species during fibre development, cDNA-SRAP (sequence-related amplified polymorphism) was applied. This technique was used to analyse genes at different stages of fibre development in G. hirsutum cv. Emian22 and G. barbadense acc. 3-79, the parents of our interspecific mapping population. A total of 4096 SRAP primer combinations were used to screen polymorphism between the DNA of the parents, and 275 highly polymorphic primers were picked out to analyse DNA and RNA from leaves and fibres at different developmental stages of the parents. A total of 168 DNA fragments were isolated from gels and sequenced: 54, 30, 38 and 41 from fibres of 5, 10, 15 and 20 days post-anthesis, respectively, and five from multi stages. To genetically map these sequences, 104 sequence-specific primers were developed and were used to screened polymorphism between the mapping parents. Finally, six markers were mapped on six chromosomes of our backbone interspecific genetic map. This work can give us a primary knowledge of differences in mechanism of fibre development between G. hirsutum and G. barbadense.
机译：陆地棉和巴巴木棉是两种栽培的四倍体棉种，其纤维质量不同。巴巴德氏菌的纤维比hirsutum的纤维更长，更强，更细。为了分离在纤维发育过程中两个物种之间表达差异的基因，使用了cDNA-SRAP（序列相关的扩增多态性）。该技术用于分析G. hirsutum cv中纤维发育不同阶段的基因。 Emian22和G. barbadense acc。 3-79，我们种间作图群体的父母。总共使用4096种SRAP引物组合来筛选亲本DNA之间的多态性，并挑选了275种高度多态性引物来分析亲本不同发育阶段的叶片和纤维中的DNA和RNA。从凝胶中分离出总共168个DNA片段，并进行了测序：分别从花后5、10、15和20天的纤维中分别提取54、30、38和41，从多阶段中提取5个。为了对这些序列进行遗传作图，开发了104种序列特异性引物，并用于筛选作图亲本之间的多态性。最后，在我们的骨干种间遗传图谱的六个染色体上绘制了六个标记。这项工作可以使我们初步了解hirsutum和G. barbadense之间的纤维发育机理。

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