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Inheritance of blast resistance and identification of SSR marker associated with it in rice cultivar RDN 98-2

机译:水稻新品种RDN 98-2的抗稻瘟病遗传及与之相关的SSR标记鉴定

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摘要

An F_2 population was developed from a cross between rice (Oryza sativa L.) genotypes, EK 70 (highly susceptible to blast) and RDN 98-2-3-5-14 (resistant to blast), to study the inheritance of blast resistance and to identify the marker associated with resistance. The F_2 population segregated in 3:1 ratio for resistance: susceptible under hot spot conditions for blast suggesting monogenic control of resistance in this population. Bulk segregant analysis conducted using a total of 25 SSR markers identified two SSRs to be polymorphic between the parents and the corresponding bulks. One of these SSR markers RM204 which has been reported to be mapped on the short arm of chromosome 6 and in close proximity of blast resistance gene/QTLs in other studies showed expected segregation ratio (1:2:1) for single gene model in the F2 population. This marker was found significantly associated with blast resistance on regression analysis.
机译:从水稻(Oryza sativa L.)基因型,EK 70(极易受稻瘟病)和RDN 98-2-3-5-14(抗稻瘟病)基因型之间的杂交中发展出F_2种群,以研究稻瘟病抗性的遗传并识别与抗性相关的标记。 F_2种群的抗药性比例为3:1:在热点条件下易受爆炸侵染,表明该种群的抗药性是单基因控制。使用总共25个SSR标记进行的批量分离分析发现,两个SSR在亲本和相应的本体之间是多态的。在其他研究中,据报道这些SSR标记RM204之一定位在6号染色体的短臂上,并靠近爆炸力抗性基因/ QTL,显示出预期的单基因模型分离率(1:2:1)。 F2人口。在回归分析中发现该标记物与抗冲击性显着相关。

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