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首页> 外文期刊>Journal of genetics >Comparative FISH mapping of BMPR1B, BMP15 and GDF9 fecundity genes on cattle, river buffalo, sheep and goat chromosomes
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Comparative FISH mapping of BMPR1B, BMP15 and GDF9 fecundity genes on cattle, river buffalo, sheep and goat chromosomes

机译：比较牛，河水牛，绵羊和山羊染色体上BMPR1B，BMP15和GDF9繁殖力基因的FISH定位

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摘要

One of the main goal in livestock genomic projects is the preparation of extensive genomic maps that identify loci affecting economically important traits. For this reason, the aim of this study was to compare several physical maps using bovine artificial chromosomes (BAC) clones containing genes related to fecundity genes. Fluorescent in situ hybridization (FISH) was performed on three gene sequences: growth differentiation factor 9 (GDF9), bone morphogenetic protein 15 (BMP15) and its type I receptor(BMPR1B). They were studied on cattle (BTA), river buffalo(BBU), sheep (OAR) and goat (CHI) for the first time. GDF9 was assigned to BTA7q22.3, BBU9q24, OAR5q22.3 and CHI7q22.3; BMP15 was assigned to BTAXq31, BBUXq36, OAR/CHIXq24; BMPR1B was assigned to BTA/OAR/CHI6q15 and BBU7q21. All loci were mapped on homologous chromosome and chromosome bands, underling the high degree of chromosome homologies among bovids and extending the cytogenetic maps of these four economically important species.
机译：牲畜基因组计划的主要目标之一是准备广泛的基因组图谱，以识别影响经济上重要特征的基因座。因此，本研究的目的是使用含有与繁殖力基因相关的基因的牛人工染色体（BAC）克隆比较几种物理图谱。对三个基因序列进行荧光原位杂交（FISH）：生长分化因子9（GDF9），骨形态发生蛋白15（BMP15）及其I型受体（BMPR1B）。首次对牛（BTA），河水牛（BBU），绵羊（OAR）和山羊（CHI）进行了研究。 GDF9被分配给BTA7q22.3，BBU9q24，OAR5q22.3和CHI7q22.3; BMP15被分配给BTAXq31，BBUXq36，OAR / CHIXq24; BMPR1B被分配给BTA / OAR / CHI6q15和BBU7q21。所有基因座都定位在同源染色体和染色体条带上，这表明牛羊之间的染色体同源性很高，并扩展了这四个经济上重要物种的细胞遗传图谱。

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