...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of genetics >AFLP studies on downy-mildew-resistant and downy-mildew-susceptible genotypes of opium poppy
【24h】

AFLP studies on downy-mildew-resistant and downy-mildew-susceptible genotypes of opium poppy

机译：AFLP研究罂粟的霜霉病抗性和霜霉病易感基因型

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Downy mildew (DM) caused by Peronospora arborescens, is a serious disease in opium poppy (Papaver somniferum), which has a world-wide spread. The establishment of DM-resistant cultivars appears to be a sustainable way to control the disease. In this paper, we present the results of a study aimed at the identification of amplified fragment length polymorphism (AFLP) markers for DM-resistance in opium poppy. Three opium poppy genotypes (inbred over about 10 years): Pps-1 (DM-resistant), Jawahar-16 (DM-susceptible) and H-9 (DM-susceptible) were crossed in a diallel manner and the F1 progeny along with the parents were subjected to AFLP analysis of chloroplast (cp) and nuclear DNA with seven and nine EcoRI / MseI primer combinations, respectively. cpDNA AFLP analysis identified 24 Pps-1 (DM-resistant)-specific unique fragments that were found to be maternally inherited in both the crosses, Pps-1 × Jawahar-16 and Pps-1 × H-9. In the case of nuclear DNA AFLP analysis, it was found that 17 fragments inherited from Pps-1 were common to the reciprocal crosses of both (i) Pps-1 and Jawahar-16 as well as (ii) Pps-1 and H-9. This is the first molecular investigation on the identification of polymorphism between DM-resistant and DM-susceptible opium poppy genotypes and development of DM-resistant opium poppy genotypespecific AFLP markers. These AFLP markers could be used in future genetic studies for analysis of linkage to the downy mildew resistance trait.
机译：霜霉病引起的霜霉病（DM）是鸦片罂粟（Papaver somniferum）中的一种严重疾病，已在世界范围内传播。建立抗DM的品种似乎是控制该病的可持续方法。在本文中，我们提出了一项旨在鉴定罂粟中DM抗性的扩增片段长度多态性（AFLP）标记的研究结果。三种鸦片罂粟基因型（近交近交系）：Pps-1（抗DM），Jawahar-16（对DM易感）和H-9（对DM易感）以双亲方式杂交，F1后代与亲本分别用7种和9种EcoRI / MseI引物组合进行叶绿体（cp）和核DNA的AFLP分析。 cpDNA AFLP分析鉴定了24个Pps-1（抗DM）特异性的独特片段，这些片段被发现在两个Pps-1×Jawahar-16和Pps-1×H-9杂交中都是母系遗传的。在核DNA AFLP分析的情况下，发现（i）Pps-1和Jawahar-16以及（ii）Pps-1和H-的反向杂交共有17个从Pps-1继承的片段9。这是鉴定抗药性和抗药性罂粟基因型之间多态性和发展抗药性罂粟基因型特异性AFLP标记的首次分子研究。这些AFLP标记可用于将来的遗传研究中，以分析与霜霉病抗性性状的连锁。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号