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首页> 外文期刊>Journal of genetics >Molecular assessment of genetic diversity in mung bean germplasm
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Molecular assessment of genetic diversity in mung bean germplasm

机译：绿豆种质遗传多样性的分子评估

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RAPD profiles were used to identify the extent of diversity among 54 accessions of mung bean that included both improved and local land races. Out of the 40 primers screened, seven primers generated 174 amplification products with an average of 24.85 bands per primer. The RAPD profiles were analysed for laccard's similarity coefficients that was found to be in the range from 0 to 0.48, indicating the presence of wide range of genetic diversity at molecular level Cluster analysis was carried out based on distances (1-similarity coefficient) using neighbour-joining method in Free Tree package The dendrogram resolved all the accessions into two major clusters, I (with 11 accessions) and II (with 43 accessions). However, the cluster was further divided into four subclusters (II A with six, II B with nine, II C with 15 and II D with 13 accessions). The distribution of the accessions in different clusters and subclusters appear es to be related to their performance in field conditions for 10 morphological traits that were scored This study indicated that the RAPD profiles provide an easy and simple technique for preliminary genetic diversity assessment of mung bean accessions that may reflect morphological trait differences among them.
机译：RAPD资料用于确定54种绿豆种的多样性程度，包括改良的和当地的土地种族。在筛选的40条引物中，有7条引物产生174个扩增产物，每个引物平均有24.85条带。分析了RAPD图谱的漆板相似系数，发现其介于0到0.48之间，表明在分子水平上存在广泛的遗传多样性。聚类分析是基于距离（1-相似系数），使用邻居树中的连接方法树状图将所有种质分解为两个主要的簇，I（11种）和II（43种）。但是，该集群又分为四个子集群（II A有六个，II B具有九个，II C具有15个，II D具有13个种）。在10个形态特征的田间条件下，不同聚类和亚类的种质分布与其表现有关。这项研究表明，RAPD谱为绿豆种质的初步遗传多样性评估提供了一种简单的技术。可能反映了它们之间的形态特征差异。

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