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首页> 外文期刊>Journal of genetics >Resynthesized Brassica juncea lines with novel organellar genome constitution obtained through protoplast fusion
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Resynthesized Brassica juncea lines with novel organellar genome constitution obtained through protoplast fusion

机译：通过原生质体融合获得具有新的细胞器基因组结构的芥菜油菜系

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摘要

Brassica juncea, a natural allotetraploid derived fromB. rapa and B. nigra, is a major oilseed crop of the Indian subcontinent. Efforts to extend its cultivation to Australia and Canada as a replacement for B. napus are being made because of its desirable features such as high-temperature tolerance, disease resistance and non-shattering silique (Woods et al. 1991). Artificial resynthesis of B. juncea has been achieved through sexual hybridization but synthesis through somatic hybridization has not been well documented. Here we report the development of B. juncea lines through protoplast fusion. RFLP analysis revealed that the hybrids inherited their plastid genome from either of the parents while the mitochondrial genome was mostly derived from B. nigra. Hybrids with mixed or recombined mitochondrial and plastid genomes were also identified. A wide variation for various agronomic traits was recorded in progeny generations. These newly resynthesized B. juncea lines hold promise for Brassica variety improvement and in heterosis breeding.
机译：芥菜油菜，来自B的天然异源四倍体。 rapa和B. nigra是印度次大陆的主要油料作物。由于其理想的特性，例如耐高温，抗病性和不破碎的角果等，正在努力将其栽培扩大到澳大利亚和加拿大，以代替甘蓝型油菜（Woods et al。1991）。芥菜型假单胞菌的人工再合成已通过有性杂交得以实现，但通过体细胞杂交的合成尚未有充分的文献记载。在这里，我们报告通过原生质体融合的芥菜型线虫的发展。 RFLP分析表明，杂种从任一亲本遗传了质体基因组，而线粒体基因组主要来自黑麦芽孢杆菌。还鉴定了具有混合或重组的线粒体和质体基因组的杂种。在子代中记录了各种农艺性状的广泛变异。这些新合成的芥菜线虫有望改善甘蓝型油菜品种和杂种优势育种。

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