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首页> 外文期刊>Journal of genetics >Development of a Y-STR 12-plex peR system and haplotype analysis in a Korean population
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Development of a Y-STR 12-plex peR system and haplotype analysis in a Korean population

机译：在韩国人口中开发Y-STR 12重peR系统和单倍型分析

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A Y-chromosomal short tandem repeat (Y-STR) dodecaplex PCR system for 12 loci has been developed, and using this system allele frequencies and haplotypes were determined in a Korean male population. From a study of 320 unrelated Korean males, 254 different haplotypes"Were identified. The haplotype diversity and discrimination capacity were estimated to be 0.996 and 0.794, respectively. Gene diversities ranged from 0.196 to 0.764 (mean gene diversity 0.557). From four loci, five mutations were identified. All cases were single repeat mutations, and mean mutation rate was determined to be 2.64 x 10-3jlocus/meiosis. The 12-plex Y-STR PCR system exhibited reliable typing results for the male-female mixed samples at ratios up to 1 : 100 and for several forensic materials from assaulted women. The system was highly sensitive even with minimal amounts of genomic DNA (0.1 ng). We believe that the newly developed 12-plex Y-STR PCR system will provide more discriminative power and will be suitable for practical forensic uses.
机译：已开发了一个用于12个基因座的Y染色体短串联重复序列（Y-STR）十二链PCR系统，并使用该系统在韩国男性人群中确定了等位基因频率和单倍型。通过对320名韩国无关男性的研究，鉴定出254个不同的单倍型。单倍型多样性和区分能力分别估计为0.996和0.794。基因多样性范围为0.196至0.764（平均基因多样性0.557）。鉴定出5个突变，所有病例均为单重复突变，平均突变率确定为2.64 x 10-3jlocus /减数分裂; 12重Y-STR PCR系统对男女混合样本按比例显示可靠的分型结果高达1：100并针对来自被殴打妇女的多种取证材料，即使使用最小量的基因组DNA（0.1 ng），该系统也是高度敏感的，我们相信新开发的12重Y-STR PCR系统将提供更大的判别能力并适合实际的法医用途。

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