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首页> 外文期刊>Journal of genetics >Comparative evaluation of genetic diversity using RAPD, SSR and cytochrome P450 gene based markers with respect to calcium content in finger millet (Eleusine coracana L. Gaertn.)
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Comparative evaluation of genetic diversity using RAPD, SSR and cytochrome P450 gene based markers with respect to calcium content in finger millet (Eleusine coracana L. Gaertn.)

机译：使用基于RAPD，SSR和基于细胞色素P450基因的标记对小米中的钙含量进行遗传多样性比较评估（Eleusine coracana L.Gaertn。）

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摘要

Genetic relationships among 52 Eleusine coracana (finger millet) genotypes collected from different districts of Uttarakhand were investigated by using randomly amplified polymorphic DNA (RAPD), simple sequence repeat (SSR) and cytochrome P450 gene based markers. A total of 18 RAPD primers, 10 SSR primers, and 10 pairs of cytochrome P450 gene based markers, respectively, revealed 49.4%, 50.2% and 58.7% polymorphism in 52 genotypes of E. coracana. Mean polymorphic information content (PIC) for each of these marker systems (0.351 for RAPD, 0.505 for SSR and 0.406 for cyt P450 gene based markers) suggested that all the marker systems were effective in determining polymorphisms. Pair-wise similarity index values ranged from 0.011 to 0.999 (RAPD), 0.010 to 0.999 (SSR) and 0.001 to 0.998 (cyt P450 gene based markers) and mean similarity index value of 0.505, 0.504 and 0.499, respectively. The dendrogram developed by RAPD, SSR and cytochrome P450 gene based primers analyses revealed that the genotypes are grouped in different clusters according to high calcium (300-450 mg/100 g), medium calcium (200-300 mg/100 g) and low calcium (100-200 mg/100 g). Mantel test employed for detection of goodness of fit established cophenetic correlation values above 0.95 for all the three marker systems. The dendrograms and principal coordinate analysis (PCA) plots derived from the binary data matrices of the three marker systems are highly concordant. High bootstrap values were obtained at major nodes of phenograms through WINBOOT software. Comparison of RAPD, SSR and cytochrome P450 gene based markers, in terms of the quality of data output, indicated that SSRs and cyt P450 gene based markers are particularly promising for the analysis of plant genome diversity. The genotypes of finger millet collected from different districts of Uttarakhand constitute a wide genetic base and clustered according to calcium contents. The identified genotypes could be used in breeding programmes and amajor input into conservation biology of cereal crops.
机译：使用随机扩增的多态性DNA（RAPD），简单序列重复（SSR）和基于细胞色素P450基因的标记物，研究了从北阿坎德邦不同地区收集的52种小隐蝇基因型之间的遗传关系。共有18种RAPD引物，10种SSR引物和10对基于细胞色素P450基因的标记，揭示了52种基因型的大肠杆菌（E. coracana）具有49.4％，50.2％和58.7％的多态性。这些标记系统中的每个标记系统的平均多态信息含量（PIC）（基于RAPD的0.351，基于SSR的0.505和基于cyt P450基因的标记的0.406）表明，所有标记系统均可有效确定多态性。成对相似性指数值的范围为0.011至0.999（RAPD），0.010至0.999（SSR）和0.001至0.998（基于Cyt P450基因的标记），平均相似性指数值分别为0.505、0.504和0.499。通过RAPD，SSR和基于细胞色素P450基因的引物分析开发的树状图显示，根据高钙（300-450 mg / 100 g），中钙（200-300 mg / 100 g）和低钙基因型将基因型分为不同的簇钙（100-200 mg / 100 g）。用于所有三个标记系统的拟合优度检测采用的Mantel测试建立了高于0.95的显色相关值。从三个标记系统的二进制数据矩阵得出的树状图和主坐标分析（PCA）图高度一致。通过WINBOOT软件，在字形图的主要节点上获得了较高的自举值。就数据输出质量而言，比较RAPD，SSR和基于细胞色素P450基因的标记，表明SSR和基于cyt P450基因的标记在分析植物基因组多样性方面特别有前途。从北阿坎德邦不同地区收集的指粟的基因型构成了广泛的遗传基础，并根据钙含量聚类。鉴定出的基因型可用于育种计划，并可为谷物作物的保护生物学提供大量投入。

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