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首页> 外文期刊>Journal of genetics >Cytosine hypomethylation at CHG and CHH sites in the pleiotropic mutants of Mendelian inheritance in Catharanthus roseus
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Cytosine hypomethylation at CHG and CHH sites in the pleiotropic mutants of Mendelian inheritance in Catharanthus roseus

机译：长春花中孟德尔遗传多效性突变体CHG和CHH位点的胞嘧啶甲基化

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摘要

The 5S and 18S rDNA sequences of Catharanthus roseus cv 'Nirmal' (wild type) and its leafless inflorescence (lli), evergreen dwarf (egd) and irregular leaf lamina (ill) single mutants and lli egd, lli ill and egd ill double mutants were characterized. The lli, egd and ill mutants of Mendelian inheritance bore the names after their most conspicuous morphological feature(s). They had been chemically induced and isolated for their salt tolerance. The double mutants were isolated as morphological segregants from crosses between single mutants. The morphological features of the two parents accompanied salt tolerance in the double mutants. All the six mutants were hypomethylated at repeat sequences, upregulated and downregulated for many genes and carried pleiotropic alterations for several traits. Here the 5S and 18S rDNAs of C. roseus were found to be relatively low in cytosine content. Cytosines were preponderantly in CG context (53%) and almost all of them were methylated(97%). The cytosines in CHH and CHG (where H = A, T or C) contexts were largely demethylated (92%) in mutants. The demethylation was attributable to reduced expression of RDR2 and DRM2 led RNA dependant DNA methylation and CMT3 led maintenance methylation pathways. Mutants had gained some cytosines by substitution of C at T sites. These perhaps arose on account of errors in DNA replication, mediated by widespread cytosine demethylation at CHG and CHH sites. It was concluded that the regulation of cytosine methylation mechanisms was disturbed in the mutants. ILL, EGD and LLI genes were identified as the positive regulators of other genes mediating the RdDM and CMT3 pathways, for establishment and maintenance of cytosine methylation in C. roseus.
机译：长春花（Catharanthus roseus cv'Nirmal'）（野生型）的5S和18S rDNA序列及其无叶的花序（lli），常绿矮人（egd）和不规则的叶片叶片（ill）单一突变体以及lli egd，lli ill和egd ill双突变体被表征。孟德尔遗传的lli，egd和ill突变体以其最显着的形态特征命名。他们被化学诱导并分离出其耐盐性。从单突变体之间的杂交中分离出双突变体作为形态分离物。两个突变体中两个亲本的形态特征伴随着耐盐性。所有六个突变体均在重复序列处被低甲基化，许多基因上调和下调，并具有多种特性的多效性改变。在此发现玫瑰色梭菌的5S和18S rDNA的胞嘧啶含量相对较低。胞嘧啶主要在CG环境中（53％），几乎全部被甲基化（97％）。 CHH和CHG（其中H = A，T或C）环境中的胞嘧啶在突变体中大部分被去甲基化（92％）。脱甲基化归因于RDR2和DRM2导致的RNA依赖性DNA甲基化和CMT3导致的维持甲基化途径的表达降低。突变体通过在T位点取代C获得了一些胞嘧啶。这些可能是由于在CHG和CHH位点广泛的胞嘧啶脱甲基作用介导的DNA复制错误所致。结论是，突变体中胞嘧啶甲基化机制的调节受到干扰。 ILL，EGD和LLI基因被鉴定为介导RdDM和CMT3途径的其他基因的正调控子，用于建立和维持玫瑰假丝酵母胞嘧啶甲基化。

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