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首页> 外文期刊>Biomolecules & therapeutics >Paraquat Induces Apoptosis through a Mitochondria-Dependent Pathway in RAW264.7 Cells
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Paraquat Induces Apoptosis through a Mitochondria-Dependent Pathway in RAW264.7 Cells

机译：百草枯通过线粒体依赖性途径在RAW264.7细胞中诱导凋亡。

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摘要

Paraquat dichloride (N,N-dimethyl-4-4'-bipiridinium, PQ) is an extremely toxic chemical that is widely used in herbicides. PQ generates reactive oxygen species (ROS) and causes multiple organ failure. In particular, PQ has been reported to be an immunotoxic agrochemical compound. PQ was shown to decrease the number of macrophages in rats and suppress monocyte phagocytic activity in mice. However, the effect of PQ on macrophage cell viability remains unclear. In this study, we evaluated the cytotoxic effect of PQ on the mouse macrophage cell line, RAW264.7 and its possible mechanism of action. RAW264.7 cells were treated with PQ (0, 75, and 150 mu M), and cellular apoptosis, mitochondrial membrane potential (MMP), and intracellular ROS levels were determined. Morphological changes to the cell nucleus and cellular apoptosis were also evaluated by DAPI and Annexin V staining, respectively. In this study, PQ induced apoptotic cell death by dose-dependently decreasing MMP. Additionally, PQ increased the cleaved form of caspase-3, an apoptotic marker. In conclusion, PQ induces apoptosis in RAW264.7 cells through a ROS-mediated mitochondrial pathway. Thus, our study improves our knowledge of PQ-induced toxicity, and may give us a greater understanding of how PQ affects the immune system.
机译：百草枯二氯化物（N，N-dimethyl-4-4'-bipiridinium，PQ）是一种剧毒化学品，广泛用于除草剂中。 PQ会产生活性氧（ROS），并导致多器官功能衰竭。特别是，据报道PQ是一种有免疫毒性的农药。 PQ可以减少大鼠巨噬细胞的数量，并抑制小鼠单核细胞的吞噬活性。但是，PQ对巨噬细胞活力的影响尚不清楚。在这项研究中，我们评估了PQ对小鼠巨噬细胞RAW264.7的细胞毒性作用及其可能的作用机制。用PQ（0、75和150μM）处理RAW264.7细胞，并测定细胞凋亡，线粒体膜电位（MMP）和细胞内ROS水平。还通过DAPI和膜联蛋白V染色分别评估了细胞核的形态学变化和细胞凋亡。在这项研究中，PQ通过剂量依赖性降低MMP诱导凋亡细胞死亡。此外，PQ增加了凋亡标记caspase-3的切割形式。总之，PQ通过ROS介导的线粒体途径诱导RAW264.7细胞凋亡。因此，我们的研究提高了我们对PQ诱导毒性的认识，并可能使我们对PQ如何影响免疫系统有更深入的了解。

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