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首页> 外文期刊>Journal of fisheries and aquatic science >Screening of Lactic Acid Bacteria from the Gut of Chrysichthys nigrodigitatus for Use as Probiotics in Aquaculture Production
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Screening of Lactic Acid Bacteria from the Gut of Chrysichthys nigrodigitatus for Use as Probiotics in Aquaculture Production

机译:从Chrysichthys nigrodigitatus肠中筛选乳酸菌作为水产养殖生产中的益生菌

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摘要

Lactic Acid Bacteria (LAB) namely LactobacillusplantarumX and Lactobacilluspentosus were isolated from the gut of Chrysichthys nigrodigitatus and identified using Bergey's manual of determinative bacteriology (API-50 CHL, BioMerieux). They are beneficial and safe organisms that improve disturbances of the indigenous microflora and host's immune system. Gut removed from fish samples were dissected and divided into 3 regions: Fore gut, mid gut and hind gut. Lactobacillus plantarum and Lactobacillus pentosus were isolated from these regions using MRS (de Man, Rogosa and Sharpe) agar and broth after subsequent culture and sub culture to obtain discrete colonies. They were characterised as Gram-positive, non-motile, catalase negative and non sporulatingbacteria.
机译:乳酸菌(LAB),即植物乳杆菌和戊糖乳杆菌,是从Chrysichthys nigrodigitatus的肠道中分离出来的,并使用Bergey的确定性细菌学手册(API-50 CHL,BioMerieux)进行鉴定。它们是有益和安全的有机体,可改善对本地微生物群和宿主免疫系统的干扰。解剖从鱼样品中取出的肠,并将其分为3个区域:前肠,中肠和后肠。在随后的培养和继代培养之后,使用MRS琼脂和肉汤从这些区域分离植物乳杆菌和戊糖乳杆菌,然后分离培养。它们的特征是革兰氏阳性,不运动,过氧化氢酶阴性和非孢子形成细菌。

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