A new device for high-pressure freezing of cultured cell monolayer using 10-microm-thin stainless discs as both culture plate and specimen carrier.

Sawaguchi A; Aoyama F; Ide S; Goto Y; Suganuma T

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A new device for high-pressure freezing of cultured cell monolayer using 10-microm-thin stainless discs as both culture plate and specimen carrier.

机译：一种使用10微米薄的不锈钢圆盘作为培养板和标本载体的高压冷冻培养细胞单层的新设备。

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High-pressure freezing (HPF) has been generally accepted as the most reliable method for cryofixation of biological samples, yielding a deep vitreous freezing. In recent cell biology, mammalian cultured cells are widely used, but HPF of cultured cell monolayer has not reached its full potential. In this study, we developed a new reliable device for HPF of cultured cell monolayer by using a 10-microm-thin stainless disc both as culture plate and specimen carrier. We describe the practical procedure, and demonstrate fine structures of HeLa cells cultured and cryofixed on the stainless discs as results.

机译：高压冷冻（HPF）已被普遍接受为生物样品冷冻固定的最可靠方法，可产生深层玻璃体冷冻。在最近的细胞生物学中，哺乳动物培养的细胞被广泛使用，但是培养的细胞单层的HPF尚未充分发挥其潜力。在这项研究中，我们通过使用10微米薄的不锈钢圆盘作为培养板和标本载体，开发了一种用于培养细胞单层HPF的新型可靠设备。我们描述了实际的过程，并证明了培养和冷冻固定在不锈钢圆盘上的HeLa细胞的优良结构。

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来源
《Journal of Electron Microscopy》 |2008年第6期|共4页
作者
Sawaguchi A; Aoyama F; Ide S; Goto Y; Suganuma T;
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正文语种 eng
中图分类电子光学仪器;
关键词
Cells; Cultured; Cryopreservation; Equipment Design; Freeze Substitution; 细胞; 培养的; 低温保藏; 设备设计; 冷冻取代; HeLa细胞; 显微镜检查; 电子; 压力;

机译：Cells;Cultured;Cryopreservation;Equipment Design;Freeze Substitution;细胞;培养的;低温保藏;设备设计;冷冻取代;HeLa细胞;显微镜检查;电子;压力;

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专利

1. A new device for high-pressure freezing of cultured cell monolayer using 10-microm-thin stainless discs as both culture plate and specimen carrier. [J] . Sawaguchi A, Aoyama F, Ide S, Journal of Electron Microscopy . 2008,第6期

机译：一种使用10微米薄的不锈钢圆盘作为培养板和标本载体的高压冷冻培养细胞单层的新设备。
2. A new approach for high-pressure freezing of primary culture cells: the fine structure and stimulation-associated transformation of cultured rabbit gastric parietal cells [J] . Sawaguchi A., McDonald KL., Karvar S., Journal of Microscopy . 2002,第Pta3期

机译：高压冷冻原代培养细胞的新方法：培养的兔胃壁细胞的精细结构和刺激相关转化
3. High-pressure freezing of plant cells cultured in cellulose microcapillaries [J] . Tiedemann J., Kollmann R., Hohenberg H. Journal of Microscopy . 1998,第Pta2期

机译：高压冷冻纤维素微毛细管中培养的植物细胞
4. Investigation of cell surface interfacial interactions of HeLa cell cultured over self-assembled monolayer surface [C] . Muhammad Mahadi Abdul Jamil, Nur Adilah Abd Rahman, Salehhuddin Hamdan, IEEE International Conference on Control System, Computing and Engineering . 2017

机译：自组装单层表面培养的HeLa细胞的细胞表面界面相互作用的研究
5. A novel superfusion device and optical imaging system to study border zone physiology in cultured heart cell monolayers. [D] . Hyatt, Christopher Jay. 2000

机译：一种新颖的融合设备和光学成像系统，用于研究培养的心脏细胞单层中的边界带生理。
6. Preparation of Cultured Cells Using High-Pressure Freezing and Freeze Substitution for Subsequent 2D or 3D Visualization in the Transmission Electron Microscope [O] . Philippa C. Hawes -1

机译：使用高压冷冻和冷冻替代技术制备培养细胞以便随后在透射电子显微镜中进行2D或3D可视化
7. Correction: 3D Cultures of Prostate Cancer Cells Cultured in a Novel High-Throughput Culture Platform Are More Resistant to Chemotherapeutics Compared to Cells Cultured in Monolayer. [O] . Karen F Chambers, Eman M O Mosaad, Pamela J Russell, 2015

机译：更正：与单层细胞培养的细胞相比，在新型高通量培养平台中培养的前列腺癌细胞的3D培养物对化学治疗药物更具抗性。

A new device for high-pressure freezing of cultured cell monolayer using 10-microm-thin stainless discs as both culture plate and specimen carrier.

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