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首页> 外文期刊>Journal of immunoassay and immunochemistry >Improved performance of ELISAs for fertility assessment using common reagents and assay protocol as evidence from quality control studies.
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Improved performance of ELISAs for fertility assessment using common reagents and assay protocol as evidence from quality control studies.

机译：作为质量控制研究的证据，使用常规试剂和测定规程改进了用于育性评估的ELISA性能。

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At our Institute, a panel of reproductive hormones, viz., estrone glucuronide (E1G), pregnanediol glucuronide (PdG), luteinising hormone (LH), and follicle stimulating hormone (FSH) are estimated by ELISA for the assessment of fertility from a single urine sample collected from a subject. In order to make the estimates less cumbersome, the selection and mode of presentation of immunoreagents of the assay were modified in such a way that, either on reconstitution or single dilution, would result in ready-to-use reagents in the assay. Retrospective analysis on the performance of these ELISAs with uniform protocols (n = 86) was compared with assays having individual assay protocols (n = 116). The performance of the assay, based on the standard curve characteristics and quality control pools, was better and the rate of acceptance of these assays improved from 87.9 to 97.6%. The simplification of assay protocols, thus, had better impact on the quality and reproducibility of immunoassay of the four analytes.
机译：在我们的研究所中，通过ELISA评估了一组生殖激素，即​​雌酮葡糖醛酸（E1G），孕烯二醇葡糖醛酸（PdG），黄体生成激素（LH）和促卵泡激素（FSH）来评估单个生殖力从受试者收集的尿液样本。为了减少估算的麻烦，对分析方法中免疫试剂的选择和呈递方式进行了修改，以使其在重构或单次稀释后都可以在分析中使用现成的试剂。将这些具有统一操作规程（n = 86）的ELISA的性能进行回顾性分析，并将其与具有单独检测规程（n = 116）的检测法进行比较。根据标准曲线特征和质量控制库，该检测的性能更好，并且这些检测的接受率从87.9提高到97.6％。因此，简化分析方法对四种分析物的免疫分析的质量和重现性具有更好的影响。

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