...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Hepatology: The Journal of the European Association for the Study of the Liver >The effects of the conserved extreme 3' end sequence of hepatitis C virus (HCV) RNA on the in vitro stabilization and translation of the HCV RNA genome.
【24h】

The effects of the conserved extreme 3' end sequence of hepatitis C virus (HCV) RNA on the in vitro stabilization and translation of the HCV RNA genome.

机译：丙型肝炎病毒（HCV）RNA保守的3'末端极端保守序列对HCV RNA基因组的体外稳定和翻译的影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BACKGROUND/AIMS: The discovery of an additional 98-base in the extreme 3' end of the hepatitis C virus (HCV) has fueled much speculation as to the role of this sequence on the behavior of the virus. It is now known that this additional 98-base sequence is present and conserved amongst HCV genotypes. This sequence is capable of forming complex and stable high-order structures that may be important in stabilizing the RNA to degradation, facilitating translation and regulating replication of the virus. We have examined the possible role of the HCV extreme 3' end sequence in stabilizing the HCV RNA genome and regulating translation in vitro. METHODS: The extreme 3' end sequence was cloned to downstream of two pre-existing two HCV clones: HCV1 (genotype 1a) and HCV-BK (genotype 1b). The reconstructed full-length clones were then tested in vitro for their stability and translation efficiency. RESULTS: We showed that the addition of the conserved 3' end sequence greatly enhanced the stability of HCV1 RNA but had only minimal effect on HCV-BK RNA in mammalian cytoplasmic extracts, suggesting that the requirements for HCV RNA stability vary amongst isolates. Following the optimization of in vitro translation conditions, it was demonstrated that the addition of this 3' end sequence did not affect the translation level from either HCV clone. CONCLUSIONS: The conserved 3' end of the HCV genome confers differential stabilizing effects on two HCV genotype 1 isolates and has no obvious role in the in vitro translation of either clone.
机译：背景/目的：在丙型肝炎病毒（HCV）的极端3'端发现了另外98个碱基的现象，引发了人们对该序列在病毒行为中的作用的猜测。现在已知在HCV基因型中存在并保守该另外的98个碱基的序列。该序列能够形成复杂而稳定的高阶结构，这对于稳定RNA的降解，促进翻译和调节病毒的复制可能非常重要。我们已经检查了HCV极端3'末端序列在稳定HCV RNA基因组和调节体外翻译中的可能作用。方法：将极端的3'末端序列克隆到两个预先存在的两个HCV克隆的下游：HCV1（基因型1a）和HCV-BK（基因型1b）。然后在体外测试重建的全长克隆的稳定性和翻译效率。结果：我们发现，保守的3'末端序列的添加大大增强了HCV1 RNA的稳定性，但对哺乳动物细胞质提取物中HCV-BK RNA的影响很小，这表明分离株之间对HCV RNA稳定性的要求有所不同。在优化体外翻译条件之后，证明了添加该3'末端序列不影响任何HCV克隆的翻译水平。结论：HCV基因组的保守3'端赋予两种HCV基因型1分离株不同的稳定作用，并且在任一克隆的体外翻译中均无明显作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号